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• W1823277855 abstract "TIA-1-related (TIAR) protein is a shuttling RNA-binding protein implicated in several steps of RNA metabolism. In the nucleus, TIAR contributes to alternative splicing events, whereas, in the cytoplasm, it acts as a translational repressor on specific transcripts such as adenine and uridine-rich element-containing mRNAs. In addition, TIAR is involved in the general translational arrest observed in cells exposed to environmental stress. This activity is encountered by the ability of TIAR to assemble abortive pre-initiation complexes coalescing into cytoplasmic granules called stress granules. To elucidate these mechanisms of translational repression, we characterized TIAR-containing complexes by tandem affinity purification followed by MS. Amongst the identified proteins, we found the splicing factor ASF/SF2, which is also present in TIA-1 protein complexes. We show that, although mostly confined in the nuclei of normal cells, ASF/SF2 migrates into stress granules upon environmental stress. The migration of ASF/SF2 into stress granules is strictly determined both by its shuttling properties and its RNA-binding capacity. Our data also indicate that ASF/SF2 down-regulates the expression of a reporter mRNA carrying adenine and uridine-rich elements within its 3′ UTR. Moreover, tethering of ASF/SF2 to a reporter transcript strongly reduces mRNA translation and stability. These results indicate that ASF/SF2 and TIA proteins cooperate in the regulation of mRNA metabolism in normal cells and in cells having to overcome environmental stress conditions. In addition, the present study provides new insights into the cytoplasmic function of ASF/SF2 and highlights mechanisms by which RNA-binding proteins regulate the diverse steps of RNA metabolism by subcellular relocalization upon extracellular stimuli. Structured digital abstract • MINT-7715509: ASF/SF2 (uniprotkb:Q6PDM2) and TIAR (uniprotkb:P70318) colocalize (MI:0403) by fluorescence microscopy (MI:0416) • MINT-7715277: TIAR (uniprotkb:P70318) physically interacts (MI:0915) with p68/Ddx5 (uniprotkb:Q61656) by anti tag coimmunoprecipitation (MI:0007) • MINT-7715293: TIAR (uniprotkb:P70318) physically interacts (MI:0915) with hnRPN M (uniprotkb:Q3THB3) by anti tag coimmunoprecipitation (MI:0007) • MINT-7715107: TIAR (uniprotkb:P70318) physically interacts (MI:0914) with hnRNP M (uniprotkb:Q3THB3), Ddx5 (uniprotkb:B1ARC0), Ddx21 (uniprotkb:Q8K2L4), ASF/SF2 (uniprotkb:Q6PDM2), Ubf1 (uniprotkb:P25976), Rps25 (uniprotkb:P62852), Rps20(uniprotkb:P60867), Rps8 (uniprotkb:P62242), Rps4 (uniprotkb:P62702), Rps3 (uniprotkb:P62908), Rpl34 (uniprotkb:Q9D1R9), Rpl31 (uniprotkb:P62900), Rpl30 (uniprotkb:P62889), Rpl23 (uniprotkb:P62830), Rpl22 (uniprotkb:P67984), Rpl21(uniprotkb:O09167), Rpl18 (uniprotkb:P35980), Rpl15 (uniprotkb:Q9CZM2), Rpl14 (uniprotkb:Q9CR57), Rpl13a (uniprotkb:P19253), Rpl13 (uniprotkb:P47963), Rpl8 (uniprotkb:P62918) and Rpl5 (uniprotkb:P47962) by tandem affinity purification(MI:0676) • MINT-7715427: TIA-1 (uniprotkb:P52912) physically interacts (MI:0915) with ASF/SF2 (uniprotkb:Q6PDM2) by anti tag coimmunoprecipitation (MI:0007) • MINT-7715264: TIAR (uniprotkb:P70318) physically interacts (MI:0915) with ASF/SF2 (uniprotkb:Q6PDM2) by anti tag coimmunoprecipitation (MI:0007) • MINT-7715309: TIAR (uniprotkb:P70318) physically interacts (MI:0915) with Ddx21 (uniprotkb:Q8K2L4) by anti tag coimmunoprecipitation (MI:0007) • MINT-7715416: TIAR (uniprotkb:P70318) physically interacts (MI:0915) with ASF/SF2 (uniprotkb:Q07955) by anti tag coimmunoprecipitation (MI:0007)" @default.
• W1823277855 created "2016-06-24" @default.
• W1823277855 creator A5005737916 @default.
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• W1823277855 creator A5033117177 @default.
• W1823277855 creator A5071503957 @default.
• W1823277855 creator A5083826470 @default.
• W1823277855 date "2010-04-30" @default.
• W1823277855 modified "2023-10-18" @default.
• W1823277855 title "The splicing factor ASF/SF2 is associated with TIA-1-related/TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression" @default.
• W1823277855 cites W1575542444 @default.
• W1823277855 cites W1964471366 @default.
• W1823277855 cites W1964571492 @default.
• W1823277855 cites W1965619464 @default.
• W1823277855 cites W1981355975 @default.
• W1823277855 cites W1989068573 @default.
• W1823277855 cites W1989504358 @default.
• W1823277855 cites W1991377750 @default.
• W1823277855 cites W1994823144 @default.
• W1823277855 cites W1996789245 @default.
• W1823277855 cites W1999037332 @default.
• W1823277855 cites W2006484928 @default.
• W1823277855 cites W2013482681 @default.
• W1823277855 cites W2015407064 @default.
• W1823277855 cites W2016493017 @default.
• W1823277855 cites W2018583668 @default.
• W1823277855 cites W2020081155 @default.
• W1823277855 cites W2024648896 @default.
• W1823277855 cites W2032527184 @default.
• W1823277855 cites W2035974120 @default.
• W1823277855 cites W2042218686 @default.
• W1823277855 cites W2045714098 @default.
• W1823277855 cites W2052137498 @default.
• W1823277855 cites W2055766587 @default.
• W1823277855 cites W2057264950 @default.
• W1823277855 cites W2057609966 @default.
• W1823277855 cites W2058176336 @default.
• W1823277855 cites W2066922827 @default.
• W1823277855 cites W2070510657 @default.
• W1823277855 cites W2076035297 @default.
• W1823277855 cites W2087744052 @default.
• W1823277855 cites W2098387574 @default.
• W1823277855 cites W2099070975 @default.
• W1823277855 cites W2100185018 @default.
• W1823277855 cites W2102018304 @default.
• W1823277855 cites W2102606027 @default.
• W1823277855 cites W2103695683 @default.
• W1823277855 cites W2106154073 @default.
• W1823277855 cites W2106767328 @default.
• W1823277855 cites W2110739636 @default.
• W1823277855 cites W2115122534 @default.
• W1823277855 cites W2123952292 @default.
• W1823277855 cites W2130137363 @default.
• W1823277855 cites W2130147634 @default.
• W1823277855 cites W2130608451 @default.
• W1823277855 cites W2132295996 @default.
• W1823277855 cites W2137614382 @default.
• W1823277855 cites W2138267133 @default.
• W1823277855 cites W2138647063 @default.
• W1823277855 cites W2139437132 @default.
• W1823277855 cites W2144333644 @default.
• W1823277855 cites W2146681915 @default.
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• W1823277855 cites W2160263647 @default.
• W1823277855 cites W2164019525 @default.
• W1823277855 cites W2172237407 @default.
• W1823277855 cites W2227746668 @default.
• W1823277855 cites W268783301 @default.
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• W1823277855 doi "https://doi.org/10.1111/j.1742-4658.2010.07664.x" @default.
• W1823277855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20477871" @default.
• W1823277855 hasPublicationYear "2010" @default.
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