FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1823773054> ?p ?o ?g. }

• W1823773054 endingPage "129" @default.
• W1823773054 startingPage "115" @default.
• W1823773054 abstract "Previously, we demonstrated that a representative M genogroup type strain of infectious haematopoietic necrosis virus (IHNV) from rainbow trout grows well in rainbow trout-derived RTG-2 cells, but a U genogroup type strain from sockeye salmon has restricted growth, associated with reduced genome replication and mRNA transcription. Here, we analysed further the mechanisms for this growth restriction of U-type IHNV in RTG-2 cells, using strategies that assessed differences in viral genes, host immune regulation and phosphorylation. To determine whether the viral glycoprotein (G) or non-virion (NV) protein was responsible for the growth restriction, four recombinant IHNV viruses were generated in which the G gene of an infectious IHNV clone was replaced by the G gene of U- or M-type IHNV and the NV gene was replaced by NV of U- or M-type IHNV. There was no significant difference in the growth of these recombinants in RTG-2 cells, indicating that G and NV proteins are not major factors responsible for the differential growth of the U- and M-type strains. Poly I:C pretreatment of RTG-2 cells suppressed the growth of both U- and M-type IHNV, although the M virus continued to replicate at a reduced level. Both viruses induced type 1 interferon (IFN1) and the IFN1 stimulated gene Mx1, but the expression levels in M-infected cells were significantly higher than in U-infected cells and an inhibitor of the IFN1-inducible protein kinase PKR, 2-aminopurine (2-AP), did not affect the growth of U- or M-type IHNV in RTG-2 cells. These data did not indicate a role for the IFN1 system in the restricted growth of U-type IHNV in RTG-2 cells. Prediction of kinase-specific phosphorylation sites in the viral phosphoprotein (P) using the NetPhosK program revealed differences between U- and M-type P genes at five phosphorylation sites. Pretreatment of RTG-2 cells with a PKC inhibitor or a p38MAPK inhibitor did not affect the growth of the U- and M-type viruses. However, 100 μm of the casein kinase II (CKII) inhibitor, 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB), reduced the titre of the U type 8.3-fold at 24 h post-infection. In contrast, 100 μm of the CKII inhibitor reduced the titre of the M type only 1.3-fold at 48 h post-infection. Our data suggest that the different growth of U- and M-type IHNV in RTG-2 cells may be linked to a differential requirement for cellular protein kinases such as CKII for their growth." @default.
• W1823773054 created "2016-06-24" @default.
• W1823773054 creator A5011460223 @default.
• W1823773054 creator A5055387148 @default.
• W1823773054 creator A5065071885 @default.
• W1823773054 creator A5069491979 @default.
• W1823773054 creator A5077553505 @default.
• W1823773054 creator A5079324448 @default.
• W1823773054 creator A5080701649 @default.
• W1823773054 date "2011-01-17" @default.
• W1823773054 modified "2023-09-28" @default.
• W1823773054 title "Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity" @default.
• W1823773054 cites W1535862536 @default.
• W1823773054 cites W1640479649 @default.
• W1823773054 cites W1691956316 @default.
• W1823773054 cites W1819850974 @default.
• W1823773054 cites W1888489312 @default.
• W1823773054 cites W1970813910 @default.
• W1823773054 cites W1970822092 @default.
• W1823773054 cites W1974811518 @default.
• W1823773054 cites W1983358040 @default.
• W1823773054 cites W1990394625 @default.
• W1823773054 cites W1997939418 @default.
• W1823773054 cites W2004363185 @default.
• W1823773054 cites W2005229360 @default.
• W1823773054 cites W2005411001 @default.
• W1823773054 cites W2007925408 @default.
• W1823773054 cites W2008731226 @default.
• W1823773054 cites W2013346825 @default.
• W1823773054 cites W2013648574 @default.
• W1823773054 cites W2027971649 @default.
• W1823773054 cites W2028149361 @default.
• W1823773054 cites W2031913105 @default.
• W1823773054 cites W2032966566 @default.
• W1823773054 cites W2035409635 @default.
• W1823773054 cites W2039591969 @default.
• W1823773054 cites W2042433752 @default.
• W1823773054 cites W2045584477 @default.
• W1823773054 cites W2050256043 @default.
• W1823773054 cites W2050963689 @default.
• W1823773054 cites W2062763734 @default.
• W1823773054 cites W2063478723 @default.
• W1823773054 cites W2068827812 @default.
• W1823773054 cites W2080291495 @default.
• W1823773054 cites W2087195143 @default.
• W1823773054 cites W2088534886 @default.
• W1823773054 cites W2095537460 @default.
• W1823773054 cites W2104244040 @default.
• W1823773054 cites W2112095981 @default.
• W1823773054 cites W2113826880 @default.
• W1823773054 cites W2114105425 @default.
• W1823773054 cites W2116294556 @default.
• W1823773054 cites W2121575693 @default.
• W1823773054 cites W2136274219 @default.
• W1823773054 cites W2137576550 @default.
• W1823773054 cites W2165381976 @default.
• W1823773054 cites W2167655650 @default.
• W1823773054 cites W2287260771 @default.
• W1823773054 cites W250068032 @default.
• W1823773054 cites W4288012 @default.
• W1823773054 doi "https://doi.org/10.1111/j.1365-2761.2010.01225.x" @default.
• W1823773054 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7194290" @default.
• W1823773054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21241319" @default.
• W1823773054 hasPublicationYear "2011" @default.
• W1823773054 type Work @default.
• W1823773054 sameAs 1823773054 @default.
• W1823773054 citedByCount "9" @default.
• W1823773054 countsByYear W18237730542012 @default.
• W1823773054 countsByYear W18237730542013 @default.
• W1823773054 countsByYear W18237730542017 @default.
• W1823773054 countsByYear W18237730542020 @default.
• W1823773054 countsByYear W18237730542021 @default.
• W1823773054 countsByYear W18237730542023 @default.
• W1823773054 crossrefType "journal-article" @default.
• W1823773054 hasAuthorship W1823773054A5011460223 @default.
• W1823773054 hasAuthorship W1823773054A5055387148 @default.
• W1823773054 hasAuthorship W1823773054A5065071885 @default.
• W1823773054 hasAuthorship W1823773054A5069491979 @default.
• W1823773054 hasAuthorship W1823773054A5077553505 @default.
• W1823773054 hasAuthorship W1823773054A5079324448 @default.
• W1823773054 hasAuthorship W1823773054A5080701649 @default.
• W1823773054 hasBestOaLocation W18237730541 @default.
• W1823773054 hasConcept C104317684 @default.
• W1823773054 hasConcept C109159458 @default.
• W1823773054 hasConcept C140704245 @default.
• W1823773054 hasConcept C153911025 @default.
• W1823773054 hasConcept C159047783 @default.
• W1823773054 hasConcept C161238802 @default.
• W1823773054 hasConcept C184235292 @default.
• W1823773054 hasConcept C2522874641 @default.
• W1823773054 hasConcept C2776178377 @default.
• W1823773054 hasConcept C2778966499 @default.
• W1823773054 hasConcept C28328180 @default.
• W1823773054 hasConcept C2909208804 @default.
• W1823773054 hasConcept C2994167347 @default.
• W1823773054 hasConcept C505870484 @default.
• W1823773054 hasConcept C54355233 @default.
• W1823773054 hasConcept C82495950 @default.

• next