Matches in SemOpenAlex for { <https://semopenalex.org/work/W182477973> ?p ?o ?g. }
- W182477973 endingPage "5421" @default.
- W182477973 startingPage "5414" @default.
- W182477973 abstract "Elements associated with the 35S promoter involved in generating the pregenomic RNA (35S RNA) of the pararetrovirus cauliflower mosaic virus have been extensively studied in heterologous systems, but little is known about their role in viral pathogenicity. To investigate these elements, premature termination codons were progressively inserted into the 3' end of the adjacent gene VI to dissect it from colinear 35S enhancer sequences. The ability to cause a systemic infection in plants was retained with loss of up to 40 amino acids from the gene VI polypeptide, but truncations into a putative zinc finger proved lethal. In the 35S promoter, removal of the TATA box also abolished infectivity. However, upstream deletions encompassing the 35S enhancer showed that the sequence between -207 and -56 from the cap site comprised nonessential elements, although complete removal of this fragment caused loss of infectivity even when domain spacing was restored by linker insertion. Two separate enhancer domains (-207 to - 150 and -95 to -56) were identified, of which either one or the other, but not both, was required for infectivity. Some mutations affected the cellular levels of viral RNAs in unexpected ways, as with removal of the as-1 enhancer element causing an increase in 35S RNA. Others altered the relative abundance of nuclear and cytoplasmic viral DNAs. Mutations in promoter domains thought to be involved in regulating tissue-specific expression did not significantly affect virus accumulation in leaves versus roots, whereas gene VI mutants showed reduced root accumulation. We conclude that elements associated with the cauliflower mosaic virus 35S promoter contain extensive nonessential regions that can behave differently in their proper context than as isolated elements." @default.
- W182477973 created "2016-06-24" @default.
- W182477973 creator A5039730385 @default.
- W182477973 creator A5060950102 @default.
- W182477973 creator A5079517968 @default.
- W182477973 date "1996-08-01" @default.
- W182477973 modified "2023-10-16" @default.
- W182477973 title "Roles of the 35S promoter and multiple overlapping domains in the pathogenicity of the pararetrovirus cauliflower mosaic virus" @default.
- W182477973 cites W1675422558 @default.
- W182477973 cites W190813562 @default.
- W182477973 cites W1969578761 @default.
- W182477973 cites W1971421846 @default.
- W182477973 cites W1977972142 @default.
- W182477973 cites W1990849226 @default.
- W182477973 cites W2000449301 @default.
- W182477973 cites W2002085357 @default.
- W182477973 cites W2008969254 @default.
- W182477973 cites W2012388553 @default.
- W182477973 cites W2016924590 @default.
- W182477973 cites W2019378493 @default.
- W182477973 cites W2019846215 @default.
- W182477973 cites W2027447633 @default.
- W182477973 cites W2029222806 @default.
- W182477973 cites W2037087376 @default.
- W182477973 cites W2043541896 @default.
- W182477973 cites W2051168894 @default.
- W182477973 cites W2055802603 @default.
- W182477973 cites W2061717420 @default.
- W182477973 cites W2067952957 @default.
- W182477973 cites W2085775305 @default.
- W182477973 cites W2088476626 @default.
- W182477973 cites W2106453505 @default.
- W182477973 cites W2129739597 @default.
- W182477973 cites W2134827727 @default.
- W182477973 cites W2154215621 @default.
- W182477973 cites W2169407808 @default.
- W182477973 cites W2171593023 @default.
- W182477973 cites W2317139279 @default.
- W182477973 cites W2336581149 @default.
- W182477973 cites W39500469 @default.
- W182477973 cites W55566338 @default.
- W182477973 cites W8138582 @default.
- W182477973 doi "https://doi.org/10.1128/jvi.70.8.5414-5421.1996" @default.
- W182477973 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/190436" @default.
- W182477973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8764052" @default.
- W182477973 hasPublicationYear "1996" @default.
- W182477973 type Work @default.
- W182477973 sameAs 182477973 @default.
- W182477973 citedByCount "21" @default.
- W182477973 countsByYear W1824779732012 @default.
- W182477973 countsByYear W1824779732021 @default.
- W182477973 crossrefType "journal-article" @default.
- W182477973 hasAuthorship W182477973A5039730385 @default.
- W182477973 hasAuthorship W182477973A5060950102 @default.
- W182477973 hasAuthorship W182477973A5079517968 @default.
- W182477973 hasBestOaLocation W1824779731 @default.
- W182477973 hasConcept C102230213 @default.
- W182477973 hasConcept C104317684 @default.
- W182477973 hasConcept C106358424 @default.
- W182477973 hasConcept C109110057 @default.
- W182477973 hasConcept C111936080 @default.
- W182477973 hasConcept C122172344 @default.
- W182477973 hasConcept C143065580 @default.
- W182477973 hasConcept C150194340 @default.
- W182477973 hasConcept C153911025 @default.
- W182477973 hasConcept C159047783 @default.
- W182477973 hasConcept C2522874641 @default.
- W182477973 hasConcept C2780037811 @default.
- W182477973 hasConcept C2780951447 @default.
- W182477973 hasConcept C54355233 @default.
- W182477973 hasConcept C67705224 @default.
- W182477973 hasConcept C86803240 @default.
- W182477973 hasConceptScore W182477973C102230213 @default.
- W182477973 hasConceptScore W182477973C104317684 @default.
- W182477973 hasConceptScore W182477973C106358424 @default.
- W182477973 hasConceptScore W182477973C109110057 @default.
- W182477973 hasConceptScore W182477973C111936080 @default.
- W182477973 hasConceptScore W182477973C122172344 @default.
- W182477973 hasConceptScore W182477973C143065580 @default.
- W182477973 hasConceptScore W182477973C150194340 @default.
- W182477973 hasConceptScore W182477973C153911025 @default.
- W182477973 hasConceptScore W182477973C159047783 @default.
- W182477973 hasConceptScore W182477973C2522874641 @default.
- W182477973 hasConceptScore W182477973C2780037811 @default.
- W182477973 hasConceptScore W182477973C2780951447 @default.
- W182477973 hasConceptScore W182477973C54355233 @default.
- W182477973 hasConceptScore W182477973C67705224 @default.
- W182477973 hasConceptScore W182477973C86803240 @default.
- W182477973 hasIssue "8" @default.
- W182477973 hasLocation W1824779731 @default.
- W182477973 hasLocation W1824779732 @default.
- W182477973 hasLocation W1824779733 @default.
- W182477973 hasLocation W1824779734 @default.
- W182477973 hasOpenAccess W182477973 @default.
- W182477973 hasPrimaryLocation W1824779731 @default.
- W182477973 hasRelatedWork W1487857142 @default.
- W182477973 hasRelatedWork W182477973 @default.
- W182477973 hasRelatedWork W1953426278 @default.