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- W1824996671 abstract "The Gram-positive soil bacterium Bacillus licheniformis is an organism of high industrial relevance. It is used in technical fermentations to produce large amounts of exoenzymes like proteases or amylases. In order to identify and remove bottlenecks in B. licheniformis-based bioprocesses, it is essential to understand the underlying metabolic pathways. Therefore, the knowledge of its complete genomic sequence is an important prerequisite for detailed physiologic analyses of this organism. In the course of this work, the complete nucleotide sequence of the wild type strain B. licheniformis DSM13/ATCC14580 was sequenced with a 7.45-fold coverage. The single circular chromosome has a total length of 4,222,748 basepairs (bp), an average G+C ratio of 46.2% and codes for 4,286 open reading frames (ORFs), 72 transfer RNAs and 7 ribosomal RNA operons. Comparative analysis of the genomic sequence of B. licheniformis DSM13 showed high functional conservation and co-linearity with respect to its close relative Bacillus subtilis. But some significant differences of the genomic properties were also identified. Many new genes of potential interest for biotechnological applications were found in B. licheniformis, such as proteases, pectate lyases, lipases and various polysaccharide degrading enzymes. With regard to the metabolism, a prominent difference is the existence of the glyoxylic acid shunt comprising the isocitrate-lyase and the malate-synthase. These enzymes enable the organism to metabolize substrates like 2,3-butanediol and acetate, which are typical end products of the overflow metabolism in bacilli during growth on carbohydrate-rich media. Additional differences include the presence of an anaerobic ribonucleotid-reductase and two type I restriction systems. A DNA microarray was constructed by PCR amplification of more than 95% of ORFs larger than 300 bp based on the genomic data of B. licheniformis DSM13/ATCC14580. The metabolism of B. licheniformis during growth on acetate and 2,3-Butanediol as single carbon source was monitored by transcriptional analysis (DNA microarray technology, growth experiments and quantitative real-time RT-PCR). Thereby, the physiological functionality of the glyoxylic acid shunt was verified, proving the validity of the bioinformatic predictions. The complete genome sequence of, and the DNA microarray for B. licheniformis DSM13, presented/described in this thesis, are important genomic tools for the optimization of commercially used B. licheniformis strains and their monitoring during industrial fermentation processes." @default.
- W1824996671 created "2016-06-24" @default.
- W1824996671 creator A5057862815 @default.
- W1824996671 date "2006-01-17" @default.
- W1824996671 modified "2023-09-24" @default.
- W1824996671 title "Genom- und Transkriptionsanalyse von Bacillus licheniformis DSM13 - einem Organismus mit großem industriellem Potential" @default.
- W1824996671 hasPublicationYear "2006" @default.
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