Matches in SemOpenAlex for { <https://semopenalex.org/work/W1825281398> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1825281398 endingPage "6" @default.
- W1825281398 startingPage "1100" @default.
- W1825281398 abstract "An existing cryopreservation method for liver slices applies 12% dimethylsulfoxide and rapid freezing. We found that cells in rat liver slices cryopreserved in this manner deteriorated rapidly upon culturing. To improve this cryopreservation method, we varied the dimethylsulfoxide concentration (0, 12, 18, and 30%), the cryopreservation medium (Williams medium E, fetal calf serum, and University of Wisconsin medium), slice thickness, and the storage period at 4 degrees C during slice preparation before cryopreservation. After thawing, slices were cultured for 4 h at 37 degrees C before their viability was evaluated by their potassium content and the number of intact cells determined histomorphologically. The biotransformation capacity of liver slices cryopreserved by the improved method was assessed by testosterone oxidation, hydroxycoumarin sulfation, and glucuronidation. Best results were obtained with 18% dimethylsulfoxide in Williams medium E: the potassium content of cryopreserved slices was higher than 65%, and the number of intact cells was higher than 60% of that in fresh slices; with 12% dimethylsulfoxide, potassium content was less than 40%, and the number of intact cells was less than 30%. Results did not differ between the three cryopreservation media. Viability of thin slices (8-10 cell layers) was better maintained than that of thicker slices (>14 cell layers). Storage at 4 degrees C of slices before cryopreservation decreased viability after cryopreservation. Both oxidative and conjugation activities were better than 60% of fresh values. Although results varied, slices cryopreserved with this improved method and cultured for 4 h retained viability between 50 and 80%, and biotransformation activity between 60 and 90% of fresh slices." @default.
- W1825281398 created "2016-06-24" @default.
- W1825281398 creator A5001597354 @default.
- W1825281398 creator A5024455168 @default.
- W1825281398 creator A5040711694 @default.
- W1825281398 creator A5058470477 @default.
- W1825281398 date "2000-09-01" @default.
- W1825281398 modified "2023-10-17" @default.
- W1825281398 title "Increased post-thaw viability and phase I and II biotransformation activity in cryopreserved rat liver slices after improvement of a fast-freezing method." @default.
- W1825281398 cites W1966321578 @default.
- W1825281398 cites W1976751084 @default.
- W1825281398 cites W1979601056 @default.
- W1825281398 cites W1990498836 @default.
- W1825281398 cites W1995913886 @default.
- W1825281398 cites W2004860494 @default.
- W1825281398 cites W2018851314 @default.
- W1825281398 cites W2036967913 @default.
- W1825281398 cites W2064015195 @default.
- W1825281398 cites W2064517350 @default.
- W1825281398 cites W2072435226 @default.
- W1825281398 cites W2086474578 @default.
- W1825281398 cites W2086495884 @default.
- W1825281398 cites W2100305360 @default.
- W1825281398 cites W2114068921 @default.
- W1825281398 cites W2133454727 @default.
- W1825281398 cites W2136697025 @default.
- W1825281398 cites W2336788452 @default.
- W1825281398 cites W2483836282 @default.
- W1825281398 cites W2500266493 @default.
- W1825281398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10950856" @default.
- W1825281398 hasPublicationYear "2000" @default.
- W1825281398 type Work @default.
- W1825281398 sameAs 1825281398 @default.
- W1825281398 citedByCount "9" @default.
- W1825281398 countsByYear W18252813982016 @default.
- W1825281398 countsByYear W18252813982023 @default.
- W1825281398 crossrefType "journal-article" @default.
- W1825281398 hasAuthorship W1825281398A5001597354 @default.
- W1825281398 hasAuthorship W1825281398A5024455168 @default.
- W1825281398 hasAuthorship W1825281398A5040711694 @default.
- W1825281398 hasAuthorship W1825281398A5058470477 @default.
- W1825281398 hasConcept C16685009 @default.
- W1825281398 hasConcept C178790620 @default.
- W1825281398 hasConcept C179933525 @default.
- W1825281398 hasConcept C181199279 @default.
- W1825281398 hasConcept C185592680 @default.
- W1825281398 hasConcept C196843134 @default.
- W1825281398 hasConcept C202751555 @default.
- W1825281398 hasConcept C2778452553 @default.
- W1825281398 hasConcept C27881333 @default.
- W1825281398 hasConcept C43617362 @default.
- W1825281398 hasConcept C517785266 @default.
- W1825281398 hasConcept C53227056 @default.
- W1825281398 hasConcept C55493867 @default.
- W1825281398 hasConcept C71924100 @default.
- W1825281398 hasConcept C86803240 @default.
- W1825281398 hasConcept C95444343 @default.
- W1825281398 hasConceptScore W1825281398C16685009 @default.
- W1825281398 hasConceptScore W1825281398C178790620 @default.
- W1825281398 hasConceptScore W1825281398C179933525 @default.
- W1825281398 hasConceptScore W1825281398C181199279 @default.
- W1825281398 hasConceptScore W1825281398C185592680 @default.
- W1825281398 hasConceptScore W1825281398C196843134 @default.
- W1825281398 hasConceptScore W1825281398C202751555 @default.
- W1825281398 hasConceptScore W1825281398C2778452553 @default.
- W1825281398 hasConceptScore W1825281398C27881333 @default.
- W1825281398 hasConceptScore W1825281398C43617362 @default.
- W1825281398 hasConceptScore W1825281398C517785266 @default.
- W1825281398 hasConceptScore W1825281398C53227056 @default.
- W1825281398 hasConceptScore W1825281398C55493867 @default.
- W1825281398 hasConceptScore W1825281398C71924100 @default.
- W1825281398 hasConceptScore W1825281398C86803240 @default.
- W1825281398 hasConceptScore W1825281398C95444343 @default.
- W1825281398 hasIssue "9" @default.
- W1825281398 hasLocation W18252813981 @default.
- W1825281398 hasOpenAccess W1825281398 @default.
- W1825281398 hasPrimaryLocation W18252813981 @default.
- W1825281398 hasRelatedWork W1825281398 @default.
- W1825281398 hasRelatedWork W1969001620 @default.
- W1825281398 hasRelatedWork W1977267727 @default.
- W1825281398 hasRelatedWork W1977364648 @default.
- W1825281398 hasRelatedWork W2025712217 @default.
- W1825281398 hasRelatedWork W2033072231 @default.
- W1825281398 hasRelatedWork W2094576757 @default.
- W1825281398 hasRelatedWork W2242145576 @default.
- W1825281398 hasRelatedWork W2370951559 @default.
- W1825281398 hasRelatedWork W4225974526 @default.
- W1825281398 hasVolume "28" @default.
- W1825281398 isParatext "false" @default.
- W1825281398 isRetracted "false" @default.
- W1825281398 magId "1825281398" @default.
- W1825281398 workType "article" @default.