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- W182544404 abstract "In the past decade, it has become increasingly apparent that in situ localization of plant molecules could lead to a better understanding of the functional activity of the plant cell during various biological processes. Recent advances in the purification of specific probes such as enzymes, lectins and antibodies and in the use of colloidal gold as a particulate marker of high electron density have provided opportunities for the development of cytochemical approaches which could not only allow an accurate localization of a wide range of plant molecules in their respective cell compartments but also help to elucidate their potential functions. Innovative developments in plant cytochemistry appear with increasing frequency and it is expected that improvements in both tissue processing and probe specificity will extend the applicability of this approach to more and more research areas in plant pathology. Because polysaccharides are major plant and microbial cell surface components, their involvement in a number of functions including recognition, attachment, and adhesion has long been recognized of key importance in the outcome of a given interaction. Consequently, their precise in situ localization has been the subject of extensive studies and a number of probes such as lectins as well as enzymes including ß-1,4-exoglucanase, 6-glucosidase, xylanase and chitinase have proved useful for studying cell surface interactions during the infection process. Another facet that has been widely investigated in recent years concerns the drastic physiological changes induced in response to infection and thought to contribute to plant disease resistance. Among the molecules involved in this altered metabolism, callose, a structural polymer of ß-1,3-glucans, and phenolic compounds have been successfully targeted by means of two newly-introduced gold-complexed enzymes: a plant ß-1,3-glucanase and a fungal laccase.The association of complementary technologies together with the refinement of existing methods and the introduction of modern approaches have undoubtedly contributed to open new avenues of investigation in the field of plant-pathogen interactions. In conjunction with biochemistry and molecular biology, cyto-and immunocytochemistry of plant tissues have proved useful for elucidating some aspects of the highly complex relationship between plants and pathogens. Thus, it is clear that both molecular and traditional approaches of plant pathology will continue to benefit from exciting, new findings generated by the in situ localization of plant and microbe molecules." @default.
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- W182544404 date "1996-01-01" @default.
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- W182544404 title "Gold Cytochemistry Applied to the Study of Plant Defense Reactions" @default.
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- W182544404 doi "https://doi.org/10.1007/978-94-009-0189-6_4" @default.
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