FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1826384807> ?p ?o ?g. }

• W1826384807 abstract "Bacterial endospores are formed in genera such as Bacillus and Clostridium in response to adverse environmental changes. Endospores have remarkable resistance to various extreme conditions and can remain dormant for extended periods of time. Clostridium spores are of particular interest due to their significant importance in several industries, such as food processing, wastewater treatment, pharmaceuticals, and health care. They are also the ideal candidates to study Panspermia and potential extraterrestrial life. However, to date, most endospore research has been conducted on Bacillus, and study of the anaerobic spore former, Clostridium, is not adequate. In this study, we have developed a general protocol to produce and purify Clostridium spores. Spectroscopy and microscopy based Endospore Viability Assay (Spectro EVA and Micro EVA) were developed and validated to assess the viability of Clostridium spores. Germinability was used as an indicator for spore viability. The basic principle of the two EVAs is to measure the release of a unique biomarker, dipicolinic acid (DPA), via germination as a proxy for endospore viability. In particular, a luminescence time-gated microscopy technique (Micro EVA) has been developed to enumerate germinable Clostridium endospores within an hour. Micro EVA is based on energy transfer from DPA to terbium ions doped in a solid matrix upon UV excitation. The distinctive emission and millisecond lifetime enables time-resolved imaging to achieve single endospore sensitivity. Comparing to traditional CFU cultivation, EVA probes the early stage of germination, resulting in a much faster detection rate (within 60 minutes) than CFU measurement (more than 3 days incubation). Micro EVA has also been successfully applied to quantify Clostridium spores in an extreme cold biosphere, Greenland ice core, and a hyper-arid biosphere, Atacama Desert, two Mars analogs on earth. The development of EVA provides a faster way to assess viability of Clostridium spores, which has significant importance in various industries. It also enables the determination of the limit and longevity of life, and provides insight on the search of extinct or extant life on Mars and other celestial bodies." @default.
• W1826384807 created "2016-06-24" @default.
• W1826384807 creator A5072206345 @default.
• W1826384807 date "2010-01-01" @default.
• W1826384807 modified "2023-09-26" @default.
• W1826384807 title "Fast Viability Assessment of Clostridium Spores: Survival in Extreme Environments" @default.
• W1826384807 cites W100436103 @default.
• W1826384807 cites W1037775133 @default.
• W1826384807 cites W115785952 @default.
• W1826384807 cites W1480109532 @default.
• W1826384807 cites W1482457750 @default.
• W1826384807 cites W1482669914 @default.
• W1826384807 cites W1506903921 @default.
• W1826384807 cites W1509390323 @default.
• W1826384807 cites W1511321848 @default.
• W1826384807 cites W1554360583 @default.
• W1826384807 cites W1556686209 @default.
• W1826384807 cites W1558880553 @default.
• W1826384807 cites W1562036311 @default.
• W1826384807 cites W1568006810 @default.
• W1826384807 cites W1569077780 @default.
• W1826384807 cites W1578216871 @default.
• W1826384807 cites W1598855973 @default.
• W1826384807 cites W165232880 @default.
• W1826384807 cites W1719764836 @default.
• W1826384807 cites W189873841 @default.
• W1826384807 cites W1939556909 @default.
• W1826384807 cites W195107636 @default.
• W1826384807 cites W1970394313 @default.
• W1826384807 cites W1970695822 @default.
• W1826384807 cites W1976489526 @default.
• W1826384807 cites W1981099026 @default.
• W1826384807 cites W1981475720 @default.
• W1826384807 cites W1984561439 @default.
• W1826384807 cites W1990362176 @default.
• W1826384807 cites W1991345054 @default.
• W1826384807 cites W1996521369 @default.
• W1826384807 cites W1998381553 @default.
• W1826384807 cites W1999405930 @default.
• W1826384807 cites W2001974944 @default.
• W1826384807 cites W2003273168 @default.
• W1826384807 cites W2006696969 @default.
• W1826384807 cites W2009265597 @default.
• W1826384807 cites W2010916714 @default.
• W1826384807 cites W2012392714 @default.
• W1826384807 cites W2018867732 @default.
• W1826384807 cites W2026573622 @default.
• W1826384807 cites W2032678548 @default.
• W1826384807 cites W2037180824 @default.
• W1826384807 cites W2039106786 @default.
• W1826384807 cites W2044451370 @default.
• W1826384807 cites W2051108092 @default.
• W1826384807 cites W2051657122 @default.
• W1826384807 cites W2059363092 @default.
• W1826384807 cites W2062134139 @default.
• W1826384807 cites W2062603102 @default.
• W1826384807 cites W2064775217 @default.
• W1826384807 cites W2068092752 @default.
• W1826384807 cites W2070965258 @default.
• W1826384807 cites W2071888538 @default.
• W1826384807 cites W2082933281 @default.
• W1826384807 cites W2084373269 @default.
• W1826384807 cites W2085262073 @default.
• W1826384807 cites W2088856953 @default.
• W1826384807 cites W2089992143 @default.
• W1826384807 cites W2090978359 @default.
• W1826384807 cites W2091311622 @default.
• W1826384807 cites W2093761988 @default.
• W1826384807 cites W2101457475 @default.
• W1826384807 cites W2103536272 @default.
• W1826384807 cites W2105018681 @default.
• W1826384807 cites W2105026937 @default.
• W1826384807 cites W2114188362 @default.
• W1826384807 cites W2114514935 @default.
• W1826384807 cites W2116705984 @default.
• W1826384807 cites W2117971049 @default.
• W1826384807 cites W2122206933 @default.
• W1826384807 cites W2127998589 @default.
• W1826384807 cites W2129995310 @default.
• W1826384807 cites W2132980978 @default.
• W1826384807 cites W2133055484 @default.
• W1826384807 cites W2134561293 @default.
• W1826384807 cites W2136273855 @default.
• W1826384807 cites W2136348906 @default.
• W1826384807 cites W2139769881 @default.
• W1826384807 cites W2140206158 @default.
• W1826384807 cites W2140775467 @default.
• W1826384807 cites W2143119917 @default.
• W1826384807 cites W2152150196 @default.
• W1826384807 cites W2154586372 @default.
• W1826384807 cites W2154690237 @default.
• W1826384807 cites W2155733474 @default.
• W1826384807 cites W2155806125 @default.
• W1826384807 cites W2156286166 @default.
• W1826384807 cites W2162109899 @default.
• W1826384807 cites W2162863229 @default.
• W1826384807 cites W2163971377 @default.
• W1826384807 cites W2164062610 @default.
• W1826384807 cites W2166361543 @default.
• W1826384807 cites W2166785990 @default.

• next