Matches in SemOpenAlex for { <https://semopenalex.org/work/W1826774080> ?p ?o ?g. }
- W1826774080 abstract "Asymmetric protein localization is essential for cell polarity and migration. We report a novel protein, Callipygian (CynA), which localizes to the lagging edge before other proteins and becomes more tightly restricted as cells polarize; additionally, it accumulates in the cleavage furrow during cytokinesis. CynA protein that is tightly localized, or clustered, to the cell rear is immobile, but when polarity is disrupted, it disperses throughout the membrane and responds to uniform chemoattractant stimulation by transiently localizing to the cytosol. These behaviors require a pleckstrin homology-domain membrane tether and a WD40 clustering domain, which can also direct other membrane proteins to the back. Fragments of CynA lacking the pleckstrin homology domain, which are normally found in the cytosol, localize to the lagging edge membrane when coexpressed with full-length protein, showing that CynA clustering is mediated by oligomerization. Cells lacking CynA have aberrant lateral protrusions, altered leading-edge morphology, and decreased directional persistence, whereas those overexpressing the protein display exaggerated features of polarity. Consistently, actin polymerization is inhibited at sites of CynA accumulation, thereby restricting protrusions to the opposite edge. We suggest that the mutual antagonism between CynA and regions of responsiveness creates a positive feedback loop that restricts CynA to the rear and contributes to the establishment of the cell axis." @default.
- W1826774080 created "2016-06-24" @default.
- W1826774080 creator A5004842912 @default.
- W1826774080 creator A5006220925 @default.
- W1826774080 creator A5066573853 @default.
- W1826774080 creator A5091366547 @default.
- W1826774080 date "2015-06-30" @default.
- W1826774080 modified "2023-10-18" @default.
- W1826774080 title "Novel protein Callipygian defines the back of migrating cells" @default.
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- W1826774080 doi "https://doi.org/10.1073/pnas.1509098112" @default.
- W1826774080 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4517219" @default.
- W1826774080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26130809" @default.
- W1826774080 hasPublicationYear "2015" @default.