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- W1827896410 abstract "This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length." @default.
- W1827896410 created "2016-06-24" @default.
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- W1827896410 date "2015-08-03" @default.
- W1827896410 modified "2023-10-16" @default.
- W1827896410 title "<b>Loss and re-establishment of desiccation tolerance in the germinated seeds of <em>Sesbania virgata</em> (Cav.) (Pers.)" @default.
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- W1827896410 doi "https://doi.org/10.4025/actasciagron.v37i3.19373" @default.
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