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W1828367075 abstract "Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate highly localized photochemistry in thick samples. Practical considerations for the application of two-photon microscopy will then be discussed, including recent technological advances. This unit will conclude with some recent applications of two-photon microscopy that highlight the key advantages over confocal microscopy and the types of experiments which would benefit most from its application." @default.
W1828367075 created "2016-06-24" @default.
W1828367075 creator A5013928213 @default.
W1828367075 creator A5036859039 @default.
W1828367075 date "2013-06-01" @default.
W1828367075 modified "2023-10-16" @default.
W1828367075 title "Two‐Photon Excitation Microscopy for the Study of Living Cells and Tissues" @default.
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W1828367075 doi "https://doi.org/10.1002/0471143030.cb0411s59" @default.
W1828367075 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4004770" @default.
W1828367075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23728746" @default.
W1828367075 hasPublicationYear "2013" @default.
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