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- W1828408802 abstract "This study aims to explore the expression and significance of ATPase in myocardium of congestive heart failure induced by pressure overload in rats.Male SD rats were divided into 3 groups randomly: Control group (C group), coarctation of abdominal aorta 15 weeks group (A group) and sham operation group (SH group). The hemodynamics parameters were measured. The size of adenine acid pool (ATP, ADP) in myocardium was measured by high performance liquid chromatography (HPLC). The mRNA copies of ATPase-α subunit, ATPase-6 subunit in ventricular myocardium were determined by RT-PCR. The protein content of F1-ATPase in mitochondria was detected by Western blot analysis.The ±dp/dtmax and contents of ATP and ADP decreased significantly in myocardium were decreased in A group. Compared with C and SH group, the mRNA copies of ATPase-α subunit, ATPase-6 subunit and the protein content of F1-ATPase were decreased in A group.The expression of F1-ATPase decreased parallel with abnormal of the adenine nucleotide in myocardium of congestive heart failure. It may play a critical role in disturbance of energy metabolism in disfunction of heart." @default.
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- W1828408802 date "2014-01-01" @default.
- W1828408802 modified "2023-09-22" @default.
- W1828408802 title "ATPase may play a critical role in disturbance of energy metabolism in congestive heart failure rats." @default.
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