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- W1829234597 abstract "The common nitrogen mustard, mechlorethamine, can form a covalent cross-link between the two bases of a cytosine–cytosine mismatch pair within a DNA duplex. The cross-linked species can be readily separated from DNA monoadducts and unreacted strands using denaturing polyacrylamide gel electrophoresis. Here, using DNA 19 mer duplexes that are mechlorethamine cross-linked at a C4–C35, C7–C32, C10–C29, or C13–C26 mismatch pair, we show that the denaturing polyacrylamide gel electrophoresis mobility of the cross-linked species is particularly sensitive to the proximity of the C–C cross-link to the duplex end. Species that are cross-linked at a C4–C35 mismatch have greater mobilities than those cross-linked at C7–C32 or C13–C26, and the species with a central C10–C29 cross-link have the lowest mobility. The mobility is also dependent on the proximity of the cross-link to a 5′-32P-phosphate or a 5′-fluorescein label. We interpret these results in terms of the conformational properties of the cross-linked species in the denaturing gel. The results are consistent with the retention of partial duplex character at the end proximal to the cross-link, with an influence on the mobility of the GC/AT ratio proximal to the cross-link and at the duplex end, and a small but discernible effect of the label." @default.
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- W1829234597 date "2013-03-01" @default.
- W1829234597 modified "2023-09-23" @default.
- W1829234597 title "Electrophoretic mobility of duplex DNA cross-linked by mechlorethamine at a cytosine-cytosine mismatch pair" @default.
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- W1829234597 doi "https://doi.org/10.1002/elps.201200543" @default.
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