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• W1829335135 abstract "A objeto de evaluar el efecto de la condicion corporal al parto (CCAP) y del nivel de alimentacion (NA) sobre la involucion uterina (IU), actividad ovarica (AO), prenez (PR) y expresion de los receptores de leptina (EXPLEP) a nivel hipotalamico y ovarico en vacas postparto, se emplearon 27 vacas mestizas ( Bos taurus x Bos indicus ). Los animales fueron asignados al azar, con arreglo factorial 2x2, a uno de cuatro tratamientos (T). T1: CCAP baja ( 2,5) + bajo NA (ACBA) (n=7) y el T4: CCAP alta (>2,5) + NA alto (ACAA) (n=7). La dieta basal fue de heno de Cynodon nlemfluensis (8% proteina cruda; PC) y un suplemento (23%; PC), a una relacion 70:30. Para evaluar IU se considero: la caracteristica de la secresion uterina (CSU), simetria de los cuernos uterinos (SCU), diametro cervical (DC) y posicion del utero (PU). La evaluacion reproductiva se realizo semanalmente desde los 15 hasta los 45 dias postparto (DPP), por palpacion transrectal, ultrasonografia y progesterona (P 4 ) en plasma. La AO fue evaluada a traves de foliculos ovaricos (F), clasificados en clases: C1 (≤5 mm), C2(6-9 mm) y C3 (≥10 mm) y la presencia de cuerpos luteos (CL). La PR fue comprobada a traves de la palpacion rectal, ultrasonido y P 4 . Cambios metabolicos energeticos fueron medidos, a traves del colesterol total (COL) y fructosamina (FRTS) a los 3, 15, 30 y 45 DPP. La expresion hipotalamica y ovarica de los receptores de leptina (EXLEP) fue evaluada mediante la tecnica de Western blot , empleando tejidos de 8 vacas sacrificadas a los 45 DPP. Los datos fueron analizados a traves de un analisis de regresion multivariado multiple, ANAVAR para medidas repetidas en el tiempo, una regresion multiple de Cox y una prueba de Kruskal y Wallis.  Los resultados muestran correlacion (P 2.5) + low FL (HBCLFL; n = 7) and; T4, high BC (>2.5) + high FL (HBCHFL; N = 7). Basal diet was made of hay from Cynodon nlemfluensis  (11% crude protein, CP) plus supplement (23% CP) at a 70:30 ratio. To assess uterine involution (UI), the following was considered: characteristic of uterine secretion (CUS), uterine horn simmetry (UHS), cervix diameter (CD), and uterine position (UP). Weekly, from 15 to 45 days postpartum (DPP), the reproductive tract was evaluated by transrectal palpation, ultrasonography, and RIA of plasma progesterone (P 4 ). The OA was assessed through the ovarian follicles (OF), which were classified as :  C1 (≤ 5 mm), C2 (6-9 mm), C3 (≥ 10 mm); and the presence of corpora lutea (CL). The PR was verified by rectal palpation, ultrasound, and  P 4 . Metabolic changes were measured as total cholesterol (TC) and fructosamine (FRTS) at 3, 15, and 45 DPP. The hypotalamic and ovarian LEPEXP were determined by Western blot from tissue samples of 8 cows slaughtered at 45 DPP. Data were statistically analyzed by multivariate multiple regressions, ANOVA for repeated measurements on time, Cox’s multiple regression and a Kruskal and Wallis test. Results show a correlation between T and C3 and between C3 and CUS at 22 and 30 DPP. Also, a correlation was detected between CL and CUS at 37 DPP. No association between C1 and T or C1 and UI was found. The multivariate multiple regressions detected a significant effect (P<0.05) of T and CD on OA. The BCAC influenced OA (P < 0.01). Cox value for CL depended on CUS and UHS (P<0.01). The effect of T and BCAC over accumulated P 4 was significant (P<0.05) and BCAC had an effect on P 4 from 30 DPP. FRTS was different among T (P<0.05), but were not among sample times. The LEPEXP for hypothalamus was higher for cows with  high BCAC, independently from FL. The PR was different among T (P<0.01). Values for PR at 120 DPP were: T3: 75% and T4: 60% vs T2: 0% and T1: 20%. In summary, T, BCAC, CUS and UHS exerted a positive effect on OA. The LEPEXP at the hypothalamus levels corroborated the response of BCAC on OA. Consequently, there was a high pregnancy rate before 120 DPP in T with better BCAC.  ( Key words: Livestock; multipurpose breeds; body condition; parturition; diet; oestrous cycle; weight gain; leptin; Aragua)" @default.
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• W1829335135 date "2008-01-01" @default.
• W1829335135 modified "2023-09-25" @default.
• W1829335135 title "Efecto de la condición corporal al parto y del nivel de alimentación sobre la involución uterina, actividad ovárica, preñez y la expresión hipotalámica y ovárica de los receptores de leptina en vacas doble propósito" @default.
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