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- W1829385510 abstract "ABSTRACT The Bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. We investigated the interplay between the sporulation temperature and the CotE morphogenetic protein in the assembly of the surface layers of B. cereus ATCC 14579 spores and on the resulting spore properties. The cotE deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°C and at the optimal growth temperature of 37°C. Transmission electron microscopy revealed that Δ cotE spores had a fragmented and detached exosporium when formed at 37°C. However, when produced at 20°C, Δ cotE spores showed defects in both coat and exosporium attachment and were susceptible to lysozyme and mutanolysin. Thus, CotE has a role in the assembly of both the coat and exosporium, which is more important during sporulation at 20°C. CotE was more represented in extracts from spores formed at 20°C than at 37°C, suggesting that increased synthesis of the protein is required to maintain proper assembly of spore surface layers at the former temperature. Δ cotE spores formed at either sporulation temperature were impaired in inosine-triggered germination and resistance to UV-C and H 2 O 2 and were less hydrophobic than wild-type (WT) spores but had a higher resistance to wet heat. While underscoring the role of CotE in the assembly of B. cereus spore surface layers, our study also suggests a contribution of the protein to functional properties of additional spore structures. Moreover, it also suggests a complex relationship between the function of a spore morphogenetic protein and environmental factors such as the temperature during spore formation." @default.
- W1829385510 created "2016-06-24" @default.
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- W1829385510 creator A5090467258 @default.
- W1829385510 date "2016-01-01" @default.
- W1829385510 modified "2023-10-14" @default.
- W1829385510 title "Sporulation Temperature Reveals a Requirement for CotE in the Assembly of both the Coat and Exosporium Layers of Bacillus cereus Spores" @default.
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- W1829385510 cites W1939280241 @default.
- W1829385510 cites W1966692594 @default.
- W1829385510 cites W1970394313 @default.
- W1829385510 cites W1972082695 @default.
- W1829385510 cites W1978163337 @default.
- W1829385510 cites W1983061635 @default.
- W1829385510 cites W1983224707 @default.
- W1829385510 cites W1984576148 @default.
- W1829385510 cites W1992732876 @default.
- W1829385510 cites W1993713722 @default.
- W1829385510 cites W1995674679 @default.
- W1829385510 cites W1998360216 @default.
- W1829385510 cites W1999797450 @default.
- W1829385510 cites W2004807551 @default.
- W1829385510 cites W2006458895 @default.
- W1829385510 cites W2013760546 @default.
- W1829385510 cites W2017993814 @default.
- W1829385510 cites W2037760480 @default.
- W1829385510 cites W2046183380 @default.
- W1829385510 cites W2054541213 @default.
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- W1829385510 cites W2087366511 @default.
- W1829385510 cites W2088522203 @default.
- W1829385510 cites W2090103138 @default.
- W1829385510 cites W2090984229 @default.
- W1829385510 cites W2097626476 @default.
- W1829385510 cites W2099417436 @default.
- W1829385510 cites W2100064973 @default.
- W1829385510 cites W2107151561 @default.
- W1829385510 cites W2107403375 @default.
- W1829385510 cites W2110313256 @default.
- W1829385510 cites W2110809377 @default.
- W1829385510 cites W2112404034 @default.
- W1829385510 cites W2114529732 @default.
- W1829385510 cites W2114698217 @default.
- W1829385510 cites W2115222934 @default.
- W1829385510 cites W2115869936 @default.
- W1829385510 cites W2117653835 @default.
- W1829385510 cites W2123687842 @default.
- W1829385510 cites W2127411075 @default.
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- W1829385510 cites W2131426382 @default.
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- W1829385510 cites W2132048208 @default.
- W1829385510 cites W2133068356 @default.
- W1829385510 cites W2137531187 @default.
- W1829385510 cites W2140617487 @default.
- W1829385510 cites W2140775467 @default.
- W1829385510 cites W2141088358 @default.
- W1829385510 cites W2144217083 @default.
- W1829385510 cites W2145997523 @default.
- W1829385510 cites W2149347333 @default.
- W1829385510 cites W2150927756 @default.
- W1829385510 cites W2151513379 @default.
- W1829385510 cites W2152194326 @default.
- W1829385510 cites W2163929699 @default.
- W1829385510 cites W2164862872 @default.
- W1829385510 cites W2165540265 @default.
- W1829385510 cites W2168009564 @default.
- W1829385510 cites W2168412090 @default.
- W1829385510 cites W2168443472 @default.
- W1829385510 cites W2169432518 @default.
- W1829385510 cites W2171800440 @default.
- W1829385510 cites W2314695949 @default.
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- W1829385510 doi "https://doi.org/10.1128/aem.02626-15" @default.
- W1829385510 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4702650" @default.
- W1829385510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26497467" @default.
- W1829385510 hasPublicationYear "2016" @default.
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