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- W1830431679 abstract "Abstract Single intraperitoneal injections (of 0.5, 1.0, and 2.0 ml per kilogram body weight) of a solution of sodium pentobarbital (50 mg/ml, in propylene glycol, 40 ml/100 ml) elevated rat serum triglyceride values obtained by the semi-automated fluorometric method of Kessler and Lederer. Increases of 267, 619, and 813%, respectively, were noted within 5 min. Liver triglycerides and other major lipid fractions in serum and liver were unaffected. The solution added directly to pooled rat sera in vitro also elevated the apparent triglyceride concentrations, propylene glycol being the responsible agent. Essentially all of the propylene glycol was recovered as apparent triglyceride. This type of chemically induced artifact can be corrected for by determining a blank on each unknown (i.e., the hydrolysis step is omitted from the procedure). Subtraction of the blank concentration from the total triglyceride concentration yields the true triglyceride concentration. Extraction of the unknown by the Folch procedure also eliminates interference from propylene glycol." @default.
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- W1830431679 date "1973-09-01" @default.
- W1830431679 modified "2023-09-26" @default.
- W1830431679 title "Propylene Glycol Interference in Determination of Serum and Liver Triglycerides" @default.
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- W1830431679 doi "https://doi.org/10.1093/clinchem/19.9.1071" @default.
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