Matches in SemOpenAlex for { <https://semopenalex.org/work/W1831932155> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1831932155 endingPage "12536" @default.
- W1831932155 startingPage "12529" @default.
- W1831932155 abstract "SEPALLATA (SEP) MADS-box genes play crucial roles in the regulation of floral growth and development. They are required for the specification of sepals, petals, stamens, and carpels as well as for floral determinacy. SEPs perform their functions through the formation of homo- or hetero-polymers, which are the molecular basis of floral quartets. In vitro assays indicated that SEP3 forms a tetramer after binding to DNA, but it is unclear whether DNA binding induces the tetramer, because SEP3 is often reported to form a dimer. Here, we analyzed the oligomeric status of SEP3 domains in the absence of the DNA-binding MADS-box domain. The truncated SEP3 was constructed as a fusion protein and expressed in prokaryotic cells. The purified protein fragment displayed as a tetramer in the size exclusion chromatographic column, and a glutaraldehyde cross-linking assay demonstrated that the protein contained a dimer unit. Yeast two-hybrid tests further verified that the fragments form homologous polymers in vivo, and that the K domain is involved in tetramer formation. Current results imply that the SEP3 protein regulates the formation of flower meristems using the tetramer as a unit, and that the DNA-binding MADS-box is dispensable for polymer formation. The C-terminal region does not contribute to homo-tetramer formation, but it may be reserved to glue other proteins." @default.
- W1831932155 created "2016-06-24" @default.
- W1831932155 creator A5007968364 @default.
- W1831932155 creator A5009016730 @default.
- W1831932155 creator A5041646127 @default.
- W1831932155 creator A5069413516 @default.
- W1831932155 creator A5072035000 @default.
- W1831932155 date "2015-01-01" @default.
- W1831932155 modified "2023-09-30" @default.
- W1831932155 title "Protein expression and characterization of SEP3 from Arabidopsis thaliana" @default.
- W1831932155 cites W1965330337 @default.
- W1831932155 cites W1970384459 @default.
- W1831932155 cites W1994104339 @default.
- W1831932155 cites W2018705472 @default.
- W1831932155 cites W2046944468 @default.
- W1831932155 cites W2047999573 @default.
- W1831932155 cites W2059291619 @default.
- W1831932155 cites W2063825344 @default.
- W1831932155 cites W2099901107 @default.
- W1831932155 cites W2100144868 @default.
- W1831932155 cites W2106214237 @default.
- W1831932155 cites W2107418677 @default.
- W1831932155 cites W2120158601 @default.
- W1831932155 cites W2120931311 @default.
- W1831932155 cites W2124471096 @default.
- W1831932155 cites W2130327038 @default.
- W1831932155 cites W2140698155 @default.
- W1831932155 cites W2143310096 @default.
- W1831932155 cites W2150892841 @default.
- W1831932155 cites W2154892216 @default.
- W1831932155 doi "https://doi.org/10.4238/2015.october.16.20" @default.
- W1831932155 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26505403" @default.
- W1831932155 hasPublicationYear "2015" @default.
- W1831932155 type Work @default.
- W1831932155 sameAs 1831932155 @default.
- W1831932155 citedByCount "3" @default.
- W1831932155 countsByYear W18319321552017 @default.
- W1831932155 countsByYear W18319321552020 @default.
- W1831932155 crossrefType "journal-article" @default.
- W1831932155 hasAuthorship W1831932155A5007968364 @default.
- W1831932155 hasAuthorship W1831932155A5009016730 @default.
- W1831932155 hasAuthorship W1831932155A5041646127 @default.
- W1831932155 hasAuthorship W1831932155A5069413516 @default.
- W1831932155 hasAuthorship W1831932155A5072035000 @default.
- W1831932155 hasBestOaLocation W18319321551 @default.
- W1831932155 hasConcept C104317684 @default.
- W1831932155 hasConcept C143065580 @default.
- W1831932155 hasConcept C153911025 @default.
- W1831932155 hasConcept C160956161 @default.
- W1831932155 hasConcept C181199279 @default.
- W1831932155 hasConcept C2775988993 @default.
- W1831932155 hasConcept C2778886173 @default.
- W1831932155 hasConcept C2779491563 @default.
- W1831932155 hasConcept C55493867 @default.
- W1831932155 hasConcept C86803240 @default.
- W1831932155 hasConcept C95444343 @default.
- W1831932155 hasConceptScore W1831932155C104317684 @default.
- W1831932155 hasConceptScore W1831932155C143065580 @default.
- W1831932155 hasConceptScore W1831932155C153911025 @default.
- W1831932155 hasConceptScore W1831932155C160956161 @default.
- W1831932155 hasConceptScore W1831932155C181199279 @default.
- W1831932155 hasConceptScore W1831932155C2775988993 @default.
- W1831932155 hasConceptScore W1831932155C2778886173 @default.
- W1831932155 hasConceptScore W1831932155C2779491563 @default.
- W1831932155 hasConceptScore W1831932155C55493867 @default.
- W1831932155 hasConceptScore W1831932155C86803240 @default.
- W1831932155 hasConceptScore W1831932155C95444343 @default.
- W1831932155 hasIssue "4" @default.
- W1831932155 hasLocation W18319321551 @default.
- W1831932155 hasLocation W18319321552 @default.
- W1831932155 hasOpenAccess W1831932155 @default.
- W1831932155 hasPrimaryLocation W18319321551 @default.
- W1831932155 hasRelatedWork W1521187372 @default.
- W1831932155 hasRelatedWork W2030501883 @default.
- W1831932155 hasRelatedWork W2043711009 @default.
- W1831932155 hasRelatedWork W2064423757 @default.
- W1831932155 hasRelatedWork W2071720405 @default.
- W1831932155 hasRelatedWork W2090851610 @default.
- W1831932155 hasRelatedWork W2310564984 @default.
- W1831932155 hasRelatedWork W2947569759 @default.
- W1831932155 hasRelatedWork W4214594088 @default.
- W1831932155 hasRelatedWork W4285140261 @default.
- W1831932155 hasVolume "14" @default.
- W1831932155 isParatext "false" @default.
- W1831932155 isRetracted "false" @default.
- W1831932155 magId "1831932155" @default.
- W1831932155 workType "article" @default.