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• W1832001947 abstract "We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacterium tumefaciens NT1RE by selection for growth on sucrose medium. This putative IS element appears to be unique to Agrobacterium strains." @default.
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• W1832001947 date "1985-11-01" @default.
• W1832001947 modified "2023-10-10" @default.
• W1832001947 title "Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria" @default.
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• W1832001947 doi "https://doi.org/10.1128/jb.164.2.918-921.1985" @default.
• W1832001947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/214340" @default.
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