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- W1832016569 abstract "At in vitro embryo production sperm selection techniques are used, one of them is Percoll® density-gradient. The aim with this study was to evaluate the plasmatic membrane (eosin-nigrosin), acrosomal (trypan blue-Giemsa; fluorescein isothiocyanate conjugated peanut agglutinin lecitin/propidium iodide (FITC-PNA/PI) and chromatin (toluidine blue) integrity, in frozen bovine spermatozoa. Twenty nine samples were analysed at post-thaw (PD) and post-Percoll® moments. Variable expressed in percentage were submitted to ANOVA and used Tuckey’s test for mean comparison. The association between variables was made by Pearson’s correlation. There were significant differences (p<0.05) between moments for motility, plasmatic membrane integrity and number of live spermatozoa with intact acrosome (FICT -PNA/PI), with higher values for PP. The percentage of cells with chromatin alteration did not differed between moments. In conclusion, Percoll® density-gradient was efficient to select a higher population of motile cells with integral plasmatic and acrosomal membranes, without alterations in nuclear chromatin condensation." @default.
- W1832016569 created "2016-06-24" @default.
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- W1832016569 date "2008-06-20" @default.
- W1832016569 modified "2023-09-24" @default.
- W1832016569 title "Seleção em gradiente de Percoll® sobre os parâmetros espermáticos do sêmen bovino congelado" @default.
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