FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W18322360> ?p ?o ?g. }

• W18322360 abstract "We have developed a novel method for studying the ability to wheat germ RNA polymerase II to initiate transcription in vitro on unnicked duplex DNA template. Our template is a plasmid which has been relaxed in vitro with wheat germ DNA topoisomerase I. The plasmid consists of SV40 cloned in pBR322. We transcribe briefly to synthesize highly labeled nascent RNA, then fractionate the resulting ternary complexes on an agarose gel to separate relaxed, closed circular DNA from other DNA species contaminating the template preparation. Under optimal conditions, we recover about half of the total RNA synthesized in complex with relaxed, closed circular DNA. The remaining RNA is in association with other DNA species or is free in solution. The principal conclusions we draw from these studies are: 1) relaxed, closed circular DNA can serve as a template for in vitro transcription, although the relative efficiencies of transcription are such that even a few per cent contamination with nicked or supercoiled template can cause transcription from these forms to dominate the reaction. 2) Initiation occurs on approximately 1 in 25 cloned circular molecules in the course of a 5-min reaction, under optimal conditions. This transcription is not predominantly from SV40 in vivo promoters and is either random or from a sufficient number of sites as to appear random. 3) There is a striking dependence of initiation frequency on purine nucleoside triphosphate concentration, especially with GTP." @default.
• W18322360 created "2016-06-24" @default.
• W18322360 creator A5051909812 @default.
• W18322360 creator A5082814655 @default.
• W18322360 date "1981-06-01" @default.
• W18322360 modified "2023-09-30" @default.
• W18322360 title "In vitro transcription by wheat germ RNA polymerase II. Initiation of RNA synthesis on relaxed, closed circular template." @default.
• W18322360 cites W1440206365 @default.
• W18322360 cites W1480065272 @default.
• W18322360 cites W1500988284 @default.
• W18322360 cites W1518390395 @default.
• W18322360 cites W1546768697 @default.
• W18322360 cites W1589911250 @default.
• W18322360 cites W1858120728 @default.
• W18322360 cites W1970977612 @default.
• W18322360 cites W1971312638 @default.
• W18322360 cites W1974077261 @default.
• W18322360 cites W1989651677 @default.
• W18322360 cites W1992091655 @default.
• W18322360 cites W1997273553 @default.
• W18322360 cites W1997506412 @default.
• W18322360 cites W2001033180 @default.
• W18322360 cites W2003636265 @default.
• W18322360 cites W2029790374 @default.
• W18322360 cites W2030551247 @default.
• W18322360 cites W2039201183 @default.
• W18322360 cites W2054829172 @default.
• W18322360 cites W2058689603 @default.
• W18322360 cites W2063450941 @default.
• W18322360 cites W2065368141 @default.
• W18322360 cites W2076369367 @default.
• W18322360 cites W2079365535 @default.
• W18322360 cites W2082287188 @default.
• W18322360 cites W2087053996 @default.
• W18322360 cites W2095254687 @default.
• W18322360 cites W2126666672 @default.
• W18322360 cites W2139614344 @default.
• W18322360 cites W2144471538 @default.
• W18322360 cites W2155330016 @default.
• W18322360 cites W2156058653 @default.
• W18322360 cites W2163671109 @default.
• W18322360 cites W4248698861 @default.
• W18322360 cites W52799549 @default.
• W18322360 doi "https://doi.org/10.1016/s0021-9258(19)69288-4" @default.
• W18322360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6263893" @default.
• W18322360 hasPublicationYear "1981" @default.
• W18322360 type Work @default.
• W18322360 sameAs 18322360 @default.
• W18322360 citedByCount "17" @default.
• W18322360 countsByYear W183223602017 @default.
• W18322360 countsByYear W183223602018 @default.
• W18322360 crossrefType "journal-article" @default.
• W18322360 hasAuthorship W18322360A5051909812 @default.
• W18322360 hasAuthorship W18322360A5082814655 @default.
• W18322360 hasBestOaLocation W183223601 @default.
• W18322360 hasConcept C104317684 @default.
• W18322360 hasConcept C134134224 @default.
• W18322360 hasConcept C138885662 @default.
• W18322360 hasConcept C153911025 @default.
• W18322360 hasConcept C179926584 @default.
• W18322360 hasConcept C199728103 @default.
• W18322360 hasConcept C2776449523 @default.
• W18322360 hasConcept C41258723 @default.
• W18322360 hasConcept C41895202 @default.
• W18322360 hasConcept C552990157 @default.
• W18322360 hasConcept C55493867 @default.
• W18322360 hasConcept C67705224 @default.
• W18322360 hasConcept C82381507 @default.
• W18322360 hasConcept C86803240 @default.
• W18322360 hasConceptScore W18322360C104317684 @default.
• W18322360 hasConceptScore W18322360C134134224 @default.
• W18322360 hasConceptScore W18322360C138885662 @default.
• W18322360 hasConceptScore W18322360C153911025 @default.
• W18322360 hasConceptScore W18322360C179926584 @default.
• W18322360 hasConceptScore W18322360C199728103 @default.
• W18322360 hasConceptScore W18322360C2776449523 @default.
• W18322360 hasConceptScore W18322360C41258723 @default.
• W18322360 hasConceptScore W18322360C41895202 @default.
• W18322360 hasConceptScore W18322360C552990157 @default.
• W18322360 hasConceptScore W18322360C55493867 @default.
• W18322360 hasConceptScore W18322360C67705224 @default.
• W18322360 hasConceptScore W18322360C82381507 @default.
• W18322360 hasConceptScore W18322360C86803240 @default.
• W18322360 hasLocation W183223601 @default.
• W18322360 hasOpenAccess W18322360 @default.
• W18322360 hasPrimaryLocation W183223601 @default.
• W18322360 hasRelatedWork W1504746908 @default.
• W18322360 hasRelatedWork W1546768697 @default.
• W18322360 hasRelatedWork W1607733014 @default.
• W18322360 hasRelatedWork W1973517823 @default.
• W18322360 hasRelatedWork W1977694232 @default.
• W18322360 hasRelatedWork W1991605266 @default.
• W18322360 hasRelatedWork W1992091655 @default.
• W18322360 hasRelatedWork W1992142056 @default.
• W18322360 hasRelatedWork W1993703453 @default.
• W18322360 hasRelatedWork W2070742012 @default.
• W18322360 hasRelatedWork W2077732194 @default.
• W18322360 hasRelatedWork W2080766484 @default.
• W18322360 hasRelatedWork W2105914656 @default.
• W18322360 hasRelatedWork W2127462625 @default.

• next