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• W1832284734 endingPage "810" @default.
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• W1832284734 abstract "To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of secreted proteins covering well-defined narrow-molecular-mass regions, or such fractions were used for lymphocyte stimulation experiments. The lymphocyte stimulation experiments showed that the fraction containing proteins with molecular masses below 10 kDa had a superior stimulating capacity in tuberculous guinea pigs whereas the 24- to 30-kDa fraction gave significantly higher skin reactions in this group compared with BCG-vaccinated guinea pigs. A precise mapping within the region from 23 to 35 kDa by using a combination of narrow overlapping fractions and purified proteins enabled the identification of the 24-kDa antigen MPT64 as a molecule specific for tuberculous infection. Thus, MPT64 is a promising candidate for a specific diagnostic skin test reagent for human tuberculosis." @default.
• W1832284734 created "2016-06-24" @default.
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• W1832284734 date "1995-03-01" @default.
• W1832284734 modified "2023-09-27" @default.
• W1832284734 title "Guinea pig cellular immune responses to proteins secreted by Mycobacterium tuberculosis" @default.
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• W1832284734 doi "https://doi.org/10.1128/iai.63.3.804-810.1995" @default.
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