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- W183313346 abstract "While a successful capsular vaccine has been developed for encapsulated serotype b haemophilus influenzae (hi), no vaccine exists for unencapsulated hi responsible for respiratory tract infections. These include otitis media that can result in sequelae in 20% of cases and remains a primary reason for pediatrician visits in developed countries. The goal of this study was to develop a system to analyze hi genes expression genome-wide during the different stages of disease. Successful comprehensive analysis at this level would provide critical insight into the pathogenesis of nthi during the course of otitis media as well as in vivo expression of particular surface antigens and thus potential candidate vaccine targets associated with infection. An initial screen of all publicly available nthi genes was carried out and conserved sequences then referenced in a database: called the hi genomic master key database. This database allows for subsequent screening of vaccine target candidates in any nthi isolate of unknown genomic sequence. All rna procedures and protocols needed for gene expression analyses were developed and adapted to the specific experimental conditions of this study. Additionally, the novel multiple consecutive lavage sampling methodology was developed to increase the amount of ex vivo bacterial mrna available for microarray analysis. This sampling method also provided insights into nthi induced otitis media with resulting clinical implications. Finally, microarray ex vivo gene expression analyses were used here for the first time to study nthi in vivo transcriptional profile genome-wide during the course of nasopharyngeal colonization and middle ear infection." @default.
- W183313346 created "2016-06-24" @default.
- W183313346 creator A5039016158 @default.
- W183313346 date "2006-01-01" @default.
- W183313346 modified "2023-09-27" @default.
- W183313346 title "Genome-wide approach to vaccine target candidates identification against otitis media causing nontypeable Haemophilus influenzae" @default.
- W183313346 hasPublicationYear "2006" @default.
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