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- W1833767359 abstract "Publisher Summary This chapter describes a rapid, sensitive fluorometric assay for avidin and biotin. The binding of biotin to avidin is accompanied by a decrease in the fluorescence intensity of the protein. These observations, combined with the known high affinity of avidin for biotin, led to the development of the assay described. Although not designed for biological fluids containing fluorescent impurities, the assay provides the most rapid and sensitive assay for samples containing relatively pure material. It is particularly convenient as commercial avidin and biotin can be used directly. A standard solution of biotin is prepared by dissolving 5 mg in 5.0 ml of buffer followed by dilution to 20 μg/ml. Two milliliters of approximately 10 μg/ml of avidin solution are pipetted into a 4 ml fluorometer cell and titrated with successive 2.0 μl aliquots of the standard 20 μg/ml biotin solution using a syringe microburette. The useful range of the biotin assay is 20 to 120 ng. Between these limits, dilution upon the addition of avidin will be less than 5%. Use of a wider emission slit may improve sensitivity." @default.
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- W1833767359 date "1979-01-01" @default.
- W1833767359 modified "2023-09-25" @default.
- W1833767359 title "[51] A rapid, sensitive fluorometric assay for avidin and biotin" @default.
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- W1833767359 doi "https://doi.org/10.1016/0076-6879(79)62231-0" @default.
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