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- W1834594789 abstract "The role of cyclin-dependent kinase inhibitors (CDKIs) is to negatively regulate cyclin-dependent kinases as a mechanism of control of cell proliferation. As such, CDKIs are being used to induce apoptosis in cancer cells to prevent their excessive reproduction. This chapter describes procedures to study apoptosis induction upon treatment with any CDKI through the evaluation of morphological and functional mitochondrial alterations, in particular, how to measure the mitochondrial membrane potential (ΔΨm) using TMRE dye, determine the content of intracellular ATP, observe mitochondrial network morphology using HeLa cells stably expressing fluorescent reporter DsRed targeting mitochondrial matrix, observe ultrastructure of the organelle using transmission electron microscopy, and, finally, assure that mitochondrial outer membrane permeabilization takes place by assessing the subcellular localization of cyt C in HeLa cells stably expressing fluorescent cyt C-GFP." @default.
- W1834594789 created "2016-06-24" @default.
- W1834594789 creator A5014661303 @default.
- W1834594789 date "2016-01-01" @default.
- W1834594789 modified "2023-09-25" @default.
- W1834594789 title "Analysis of CDK Inhibitor Action on Mitochondria-Mediated Apoptosis" @default.
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- W1834594789 doi "https://doi.org/10.1007/978-1-4939-2926-9_9" @default.
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