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- W1835059569 abstract "The convergence point of growth regulatory pathways that control cell proliferation is the initiation of genome replication, the core of which is the assembly of pre-replicative complexes resulting in chromatin being ‘licensed’ for DNA replication in the subsequent S phase. We have analysed regulation of the pre-replicative complex proteins ORC, Cdc6, and MCM in cycling and non-proliferating quiescent, differentiated and replicative senescent human cells. Moreover, a human cell-free DNA replication system has been exploited to study the replicative capacity of nuclei and cytosolic extracts prepared from these cells. These studies demonstrate that downregulation of the Cdc6 and MCM constituents of the replication initiation pathway is a common downstream mechanism for loss of proliferative capacity in human cells. Furthermore, analysis of MCM protein expression in self-renewing, stable and permanent human tissues shows that the three classes of tissue have developed very different growth control strategies with respect to replication licensing. Notably, in breast tissue we found striking differences between the proportion of mammary acinar cells that express MCM proteins and those labelled with conventional proliferation markers, raising the intriguing possibility that progenitor cells of some tissues are held in a prolonged G1 phase or ‘in-cycle arrest’. We conclude that biomarkers for replication-licensed cells detect, in addition to actively proliferating cells, cells with growth potential, a concept that has major implications for developmental and cancer biology." @default.
- W1835059569 created "2016-06-24" @default.
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- W1835059569 date "2001-06-01" @default.
- W1835059569 modified "2023-10-16" @default.
- W1835059569 title "DNA replication licensing and human cell proliferation" @default.
- W1835059569 cites W1266969623 @default.
- W1835059569 cites W142764342 @default.
- W1835059569 cites W1481637008 @default.
- W1835059569 cites W1514540426 @default.
- W1835059569 cites W1965076280 @default.
- W1835059569 cites W1966453709 @default.
- W1835059569 cites W1966826646 @default.
- W1835059569 cites W1968863900 @default.
- W1835059569 cites W1974208182 @default.
- W1835059569 cites W1974391793 @default.
- W1835059569 cites W1977006573 @default.
- W1835059569 cites W1979895440 @default.
- W1835059569 cites W1982453928 @default.
- W1835059569 cites W1982532503 @default.
- W1835059569 cites W1985597947 @default.
- W1835059569 cites W1985934339 @default.
- W1835059569 cites W1986400206 @default.
- W1835059569 cites W1992934869 @default.
- W1835059569 cites W2001095597 @default.
- W1835059569 cites W2001479945 @default.
- W1835059569 cites W2002372083 @default.
- W1835059569 cites W2004314923 @default.
- W1835059569 cites W2008136020 @default.
- W1835059569 cites W2009492491 @default.
- W1835059569 cites W2011161974 @default.
- W1835059569 cites W2012116865 @default.
- W1835059569 cites W2017816163 @default.
- W1835059569 cites W2018782003 @default.
- W1835059569 cites W2025011614 @default.
- W1835059569 cites W2027308884 @default.
- W1835059569 cites W2029080338 @default.
- W1835059569 cites W2032141702 @default.
- W1835059569 cites W2036385196 @default.
- W1835059569 cites W2038329703 @default.
- W1835059569 cites W2039396493 @default.
- W1835059569 cites W2044279758 @default.
- W1835059569 cites W2046286320 @default.
- W1835059569 cites W2047384838 @default.
- W1835059569 cites W2048719426 @default.
- W1835059569 cites W2050339248 @default.
- W1835059569 cites W2050551830 @default.
- W1835059569 cites W2050796617 @default.
- W1835059569 cites W2052381578 @default.
- W1835059569 cites W2053437486 @default.
- W1835059569 cites W2053717292 @default.
- W1835059569 cites W2058023641 @default.
- W1835059569 cites W2058967103 @default.
- W1835059569 cites W2066079797 @default.
- W1835059569 cites W2066607538 @default.
- W1835059569 cites W2070230428 @default.
- W1835059569 cites W2078239174 @default.
- W1835059569 cites W2080153803 @default.
- W1835059569 cites W2083523041 @default.
- W1835059569 cites W2085061139 @default.
- W1835059569 cites W2085158911 @default.
- W1835059569 cites W2086120184 @default.
- W1835059569 cites W2086598375 @default.
- W1835059569 cites W2087898360 @default.
- W1835059569 cites W2088612272 @default.
- W1835059569 cites W2088628455 @default.
- W1835059569 cites W2090178205 @default.
- W1835059569 cites W2090985010 @default.
- W1835059569 cites W2092220124 @default.
- W1835059569 cites W2093703127 @default.
- W1835059569 cites W2105022572 @default.
- W1835059569 cites W2108482850 @default.
- W1835059569 cites W2121619780 @default.
- W1835059569 cites W2130258750 @default.
- W1835059569 cites W2134049041 @default.
- W1835059569 cites W2139537685 @default.
- W1835059569 cites W2142867416 @default.
- W1835059569 cites W2145475006 @default.
- W1835059569 cites W2150948143 @default.
- W1835059569 cites W2155643713 @default.
- W1835059569 cites W2163386603 @default.
- W1835059569 cites W2165155144 @default.
- W1835059569 cites W2167630842 @default.
- W1835059569 cites W2168215139 @default.
- W1835059569 cites W2170085122 @default.
- W1835059569 cites W2188497916 @default.
- W1835059569 cites W2308586243 @default.
- W1835059569 cites W2410663118 @default.
- W1835059569 cites W2747293140 @default.
- W1835059569 cites W2886664032 @default.
- W1835059569 cites W4237172887 @default.