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• W1835133731 startingPage "71" @default.
• W1835133731 abstract "Aims: To determine the occurrence and proportion of Escherichia coli O157:H7 in faeces, skin swabs and carcasses before and after washing, from sheep and goats in Ethiopia. Method and Results: Individual samples were enriched in modified tryptic soy broth with novobiocin, concentrated using immunomagnetic separation (IMS) and plated onto cefixime-tellurite containing sorbitol MacConkey agar. Presumptive colonies were confirmed by biochemical tests and subjected to latex agglutination tests. A PCR was performed on isolates for the detection of stx1, stx2 and eae genes. Escherichia coli O157:H7 was isolated from faeces (4·7%), skin swabs (8·7%) and carcasses before washing (8·1%) and after washing (8·7%) and on water samples (4·2%). The proportion of carcasses contaminated with E. coli O157:H7 was strongly associated with those recovered from faecal and skin samples. Both stx1 and stx2 genes were identified from one E. coli O157:H7 isolate from a goat carcass. Conclusions: Even though the numbers of samples examined in this study were limited to one abattoir, sheep and goats can be potential sources of E. coli O157:H7 for human infection in the country. Control measures to reduce the public health risks arising from E. coli O157:H7 in reservoir animals need to be addressed at abattoir levels by reducing skin and faecal sources and carcass contaminations at different stages of slaughter operations. Significance and Impact of the Study: Escherichia coli O157:H7 was detected from carcasses before and after washing during slaughtering operations, and one O157 isolate was positive for verotoxins." @default.
• W1835133731 created "2016-06-24" @default.
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• W1835133731 date "2010-01-01" @default.
• W1835133731 modified "2023-09-27" @default.
• W1835133731 title "Occurrence of Escherichia coli O157:H7 in faeces, skin and carcasses from sheep and goats in Ethiopia" @default.
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• W1835133731 doi "https://doi.org/10.1111/j.1472-765x.2009.02757.x" @default.
• W1835133731 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19895421" @default.
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