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- W1835431368 abstract "Six-week-old SPF BALB/c mice were immunized with purified bluetongue virus(BTV) emulsified with Freund's adjuvant.The spleen cells isolated from vaccinated mice were fused with SP2/0 cells.Three hybridoma of 1F5,4E5 and 6A1,screened using ELISA,BTV VP7-ELISA and BHK-ELISA could secrete stably specific antibodies to VP7 protein of BTV.A double antibody sandwich ELISA(DAS-ELISA) for detection of BTV was established based on monoclonal antibody(1F5) and rabbit-anti-BTV polyclonal antibody.Positive samples with BTV or epizootic haemorrhagic disease virus of deer,vesicular stomatitis virus,Akabane disease virus and Peste des petits ruminants virus were examined by the established ELISA,respectively.The result showed that DAS-ELISA had good specificity.Then DAS-ELISA was used to detect 259 clinical samples,and RT-PCR was used as a reference method.The results showed that the specificity and sensitivity of DAS-ELISA were 99.1% and 85.7%,respectively,and the agreement ratio of DAS-ELISA to RT-PCR was 97.7%.These results indicated that DAS-ELISA was suitable for rapid detection and epidemiological investigation of BTV infection in ruminants." @default.
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- W1835431368 date "2009-01-01" @default.
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- W1835431368 title "Development of double antibody sandwich ELISA for detection of bluetongue virus" @default.
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