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- W1836240501 abstract "Abstract Investigation of the role of the polypeptide chain elongation factor 3 (EF-3) of yeast indicates that EF-3 participates in the elongation cycle by stimulating the function of EF-1 alpha in binding aminoacyl-tRNA (aa-tRNA) to the ribosome. In the yeast system, the binding of the ternary complex of EF-1 alpha.GTP.aa-tRNA to the ribosome is stoichiometric to the amount of EF-1 alpha. In the presence of EF-3, EF-1 alpha functions catalytically in the above mentioned reaction. The EF-3 effect is manifest in the presence of ATP, GTP, or ITP. A nonhydrolyzable analog of ATP does not replace ATP in this reaction, indicating a role of ATP hydrolysis in EF-3 function. The stimulatory effect of EF-3 is, in many respects, distinct from that of EF-1 beta. Factor 3 does not stimulate the formation of a binary complex between EF-1 alpha and GTP, nor does it stimulate the exchange of EF-1 alpha-bound GDP with free GTP. The formation of a ternary complex between EF-1 alpha.GTP.aa-tRNA is also not affected by EF-3. It appears that the only reaction of the elongation cycle that is stimulated by EF-3 is EF-1 alpha-dependent binding of aa-tRNA to the ribosome. Purified elongation factor 3, isolated from a temperature-sensitive mutant, failed to stimulate this reaction after exposure to a nonpermissive temperature. A heterologous combination of ribosomal subunits from yeast and wheat germ manifest the requirement for EF-3, dependent upon the source of the ribosomal subunit. A combination of 40 S subunits from yeast and 60 from wheat germ showed the stimulatory effect of EF-3 in polyphenylalanine synthesis (Chakraburtty, K., and Kamath, A. (1988) Int. J. Biochem. 20, 581-590). However, we failed to demonstrate the effect of EF-3 in binding aa-tRNA to such a heterologous combination of the ribosomal subunits." @default.
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- W1836240501 date "1989-09-01" @default.
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- W1836240501 title "Role of yeast elongation factor 3 in the elongation cycle" @default.
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- W1836240501 doi "https://doi.org/10.1016/s0021-9258(19)84845-7" @default.
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