Matches in SemOpenAlex for { <https://semopenalex.org/work/W1836672409> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1836672409 abstract "We reported a simple and efficient method, which combines restriction endonuclease digestion and adaptor ligation, for cloning unknown genomic sequences adjacent to a known sequence. After total genomic DNA is completely digested with the different sticky-end restriction endonuclease separately, the ends are full. The DNA fragments with blunt-end were then ligated separately to the adaptor. The adaptor-ligated genomic DNA fragments are used as template for cloning flanking regions from all sequence of interest. A first round PCR is performed with a gene-specific primer and the adaptor primer at its 5’ and 3’ end. This is followed by second PCR amplification with a nested gene-specific primer and the nested adaptor primer. Finally, the amplified products are fractionated, cloned, and sequenced. Using this method, we cloned the 5’ and 3’ flanking region of a mannose-binding lectin gene based upon DNA fragment obtained from China Crinum (Crinum asiaticum var. (Roxb. ex Herb.) Barker)." @default.
- W1836672409 created "2016-06-24" @default.
- W1836672409 creator A5054119365 @default.
- W1836672409 creator A5063940173 @default.
- W1836672409 creator A5079121135 @default.
- W1836672409 creator A5087532194 @default.
- W1836672409 date "2008-01-01" @default.
- W1836672409 modified "2023-09-24" @default.
- W1836672409 title "Cloning and analysis of the 5’ and 3’ flanking regions of the Crinum asiaticum agglutinin gene by genomic walking" @default.
- W1836672409 cites W1546448925 @default.
- W1836672409 cites W1968638592 @default.
- W1836672409 cites W1969304546 @default.
- W1836672409 cites W1975782634 @default.
- W1836672409 cites W1987849047 @default.
- W1836672409 cites W1987992233 @default.
- W1836672409 cites W2021674227 @default.
- W1836672409 cites W2039847706 @default.
- W1836672409 cites W2045348184 @default.
- W1836672409 cites W2054696325 @default.
- W1836672409 cites W2080491926 @default.
- W1836672409 cites W2080600748 @default.
- W1836672409 cites W2103513904 @default.
- W1836672409 cites W2168518636 @default.
- W1836672409 cites W2735566229 @default.
- W1836672409 cites W2935873247 @default.
- W1836672409 cites W46342175 @default.
- W1836672409 hasPublicationYear "2008" @default.
- W1836672409 type Work @default.
- W1836672409 sameAs 1836672409 @default.
- W1836672409 citedByCount "1" @default.
- W1836672409 crossrefType "journal-article" @default.
- W1836672409 hasAuthorship W1836672409A5054119365 @default.
- W1836672409 hasAuthorship W1836672409A5063940173 @default.
- W1836672409 hasAuthorship W1836672409A5079121135 @default.
- W1836672409 hasAuthorship W1836672409A5087532194 @default.
- W1836672409 hasConcept C104317684 @default.
- W1836672409 hasConcept C121050878 @default.
- W1836672409 hasConcept C128319531 @default.
- W1836672409 hasConcept C153911025 @default.
- W1836672409 hasConcept C17757408 @default.
- W1836672409 hasConcept C178790620 @default.
- W1836672409 hasConcept C185592680 @default.
- W1836672409 hasConcept C199360897 @default.
- W1836672409 hasConcept C2777028655 @default.
- W1836672409 hasConcept C2777563447 @default.
- W1836672409 hasConcept C41008148 @default.
- W1836672409 hasConcept C54355233 @default.
- W1836672409 hasConcept C552990157 @default.
- W1836672409 hasConcept C86803240 @default.
- W1836672409 hasConceptScore W1836672409C104317684 @default.
- W1836672409 hasConceptScore W1836672409C121050878 @default.
- W1836672409 hasConceptScore W1836672409C128319531 @default.
- W1836672409 hasConceptScore W1836672409C153911025 @default.
- W1836672409 hasConceptScore W1836672409C17757408 @default.
- W1836672409 hasConceptScore W1836672409C178790620 @default.
- W1836672409 hasConceptScore W1836672409C185592680 @default.
- W1836672409 hasConceptScore W1836672409C199360897 @default.
- W1836672409 hasConceptScore W1836672409C2777028655 @default.
- W1836672409 hasConceptScore W1836672409C2777563447 @default.
- W1836672409 hasConceptScore W1836672409C41008148 @default.
- W1836672409 hasConceptScore W1836672409C54355233 @default.
- W1836672409 hasConceptScore W1836672409C552990157 @default.
- W1836672409 hasConceptScore W1836672409C86803240 @default.
- W1836672409 hasIssue "20" @default.
- W1836672409 hasLocation W18366724091 @default.
- W1836672409 hasOpenAccess W1836672409 @default.
- W1836672409 hasPrimaryLocation W18366724091 @default.
- W1836672409 hasRelatedWork W1549277556 @default.
- W1836672409 hasRelatedWork W1977893584 @default.
- W1836672409 hasRelatedWork W1992448715 @default.
- W1836672409 hasRelatedWork W1994998449 @default.
- W1836672409 hasRelatedWork W2001656101 @default.
- W1836672409 hasRelatedWork W2009134744 @default.
- W1836672409 hasRelatedWork W2010367544 @default.
- W1836672409 hasRelatedWork W2013754126 @default.
- W1836672409 hasRelatedWork W2040170875 @default.
- W1836672409 hasRelatedWork W2047652106 @default.
- W1836672409 hasRelatedWork W2050831724 @default.
- W1836672409 hasRelatedWork W2092151058 @default.
- W1836672409 hasRelatedWork W2361773463 @default.
- W1836672409 hasRelatedWork W2365900525 @default.
- W1836672409 hasRelatedWork W2369888075 @default.
- W1836672409 hasRelatedWork W2389352178 @default.
- W1836672409 hasRelatedWork W2406187073 @default.
- W1836672409 hasRelatedWork W2412830053 @default.
- W1836672409 hasRelatedWork W3148895713 @default.
- W1836672409 hasRelatedWork W882107180 @default.
- W1836672409 hasVolume "7" @default.
- W1836672409 isParatext "false" @default.
- W1836672409 isRetracted "false" @default.
- W1836672409 magId "1836672409" @default.
- W1836672409 workType "article" @default.