FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1836699491> ?p ?o ?g. }

• W1836699491 endingPage "7603" @default.
• W1836699491 startingPage "7592" @default.
• W1836699491 abstract "Multiple heat shock transcription factors (HSFs) have been discovered in several higher eukaryotes, raising questions about their respective functions in the cellular stress response. Previously, we had demonstrated that the two mouse HSFs (mHSF1 and mHSF2) interacted differently with the HSP70 heat shock element (HSE). To further address the issues of cooperativity and the interaction of multiple HSFs with the HSE, we selected new mHSF1 and mHSF2 DNA-binding sites through protein binding and PCR amplification. The selected sequences, isolated from a random population, were composed primarily of alternating inverted arrays of the pentameric consensus 5'-nGAAn-3', and the nucleotides flanking the core GAA motif were nonrandom. The average number of pentamers selected in each binding site was four to five for mHSF1 and two to three for mHSF2, suggesting differences in the potential for cooperative interactions between adjacent trimers. Our comparison of mHSF1 and mHSF2 binding to selected sequences further substantiated these differences in cooperativity as mHSF1, unlike mHSF2, was able to bind to extended HSE sequences, confirming previous observations on the HSP70 HSE. Certain selected sequences that exhibited preferential binding of mHSF1 or mHSF2 were mutagenized, and these studies demonstrated that the affinity of an HSE for a particular HSF and the extent of HSF interaction could be altered by single base substitutions. The domain of mHSF1 utilized for cooperative interactions was transferable, as chimeric mHSF1/mHSF2 proteins demonstrated that sequences within or adjacent to the mHSF1 DNA-binding domain were responsible. We have demonstrated that HSEs can have a greater affinity for a specific HSF and that in mice, mHSF1 utilizes a higher degree of cooperativity in DNA binding. This suggests two ways in which cells have developed to regulate the activity of closely related transcription factors: developing the ability to fully occupy the target binding site and alteration of the target site to favor interaction with a specific factor." @default.
• W1836699491 created "2016-06-24" @default.
• W1836699491 creator A5004866198 @default.
• W1836699491 creator A5065061503 @default.
• W1836699491 date "1994-11-01" @default.
• W1836699491 modified "2023-10-11" @default.
• W1836699491 title "Selection of new HSF1 and HSF2 DNA-binding sites reveals difference in trimer cooperativity." @default.
• W1836699491 cites W1500674875 @default.
• W1836699491 cites W1508410554 @default.
• W1836699491 cites W1526483332 @default.
• W1836699491 cites W1544887968 @default.
• W1836699491 cites W1598830628 @default.
• W1836699491 cites W1747840667 @default.
• W1836699491 cites W1781453437 @default.
• W1836699491 cites W1821289342 @default.
• W1836699491 cites W1825292303 @default.
• W1836699491 cites W1830116125 @default.
• W1836699491 cites W1954249603 @default.
• W1836699491 cites W1973445420 @default.
• W1836699491 cites W1980014349 @default.
• W1836699491 cites W1981765089 @default.
• W1836699491 cites W1992021393 @default.
• W1836699491 cites W2001610972 @default.
• W1836699491 cites W2005131012 @default.
• W1836699491 cites W2006609702 @default.
• W1836699491 cites W2012015460 @default.
• W1836699491 cites W2013683245 @default.
• W1836699491 cites W2019673480 @default.
• W1836699491 cites W2020257254 @default.
• W1836699491 cites W2021866483 @default.
• W1836699491 cites W2032849217 @default.
• W1836699491 cites W2040385763 @default.
• W1836699491 cites W2041309113 @default.
• W1836699491 cites W2048438868 @default.
• W1836699491 cites W2093417376 @default.
• W1836699491 cites W2116608195 @default.
• W1836699491 cites W2119624960 @default.
• W1836699491 cites W2125724889 @default.
• W1836699491 cites W2127554597 @default.
• W1836699491 cites W2131287384 @default.
• W1836699491 cites W2150785606 @default.
• W1836699491 cites W2155501916 @default.
• W1836699491 cites W2159271990 @default.
• W1836699491 cites W2163964409 @default.
• W1836699491 cites W2170064419 @default.
• W1836699491 cites W2178492699 @default.
• W1836699491 cites W958315937 @default.
• W1836699491 doi "https://doi.org/10.1128/mcb.14.11.7592" @default.
• W1836699491 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/359295" @default.
• W1836699491 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7935474" @default.
• W1836699491 hasPublicationYear "1994" @default.
• W1836699491 type Work @default.
• W1836699491 sameAs 1836699491 @default.
• W1836699491 citedByCount "116" @default.
• W1836699491 countsByYear W18366994912012 @default.
• W1836699491 countsByYear W18366994912013 @default.
• W1836699491 countsByYear W18366994912014 @default.
• W1836699491 countsByYear W18366994912015 @default.
• W1836699491 countsByYear W18366994912016 @default.
• W1836699491 countsByYear W18366994912017 @default.
• W1836699491 countsByYear W18366994912018 @default.
• W1836699491 countsByYear W18366994912020 @default.
• W1836699491 countsByYear W18366994912021 @default.
• W1836699491 countsByYear W18366994912022 @default.
• W1836699491 crossrefType "journal-article" @default.
• W1836699491 hasAuthorship W1836699491A5004866198 @default.
• W1836699491 hasAuthorship W1836699491A5065061503 @default.
• W1836699491 hasBestOaLocation W18366994912 @default.
• W1836699491 hasConcept C104317684 @default.
• W1836699491 hasConcept C107824862 @default.
• W1836699491 hasConcept C153911025 @default.
• W1836699491 hasConcept C166014724 @default.
• W1836699491 hasConcept C178790620 @default.
• W1836699491 hasConcept C185581394 @default.
• W1836699491 hasConcept C185592680 @default.
• W1836699491 hasConcept C187250156 @default.
• W1836699491 hasConcept C205260736 @default.
• W1836699491 hasConcept C2776403692 @default.
• W1836699491 hasConcept C2779546866 @default.
• W1836699491 hasConcept C52981337 @default.
• W1836699491 hasConcept C54355233 @default.
• W1836699491 hasConcept C552990157 @default.
• W1836699491 hasConcept C68991219 @default.
• W1836699491 hasConcept C86339819 @default.
• W1836699491 hasConcept C86803240 @default.
• W1836699491 hasConcept C94966510 @default.
• W1836699491 hasConceptScore W1836699491C104317684 @default.
• W1836699491 hasConceptScore W1836699491C107824862 @default.
• W1836699491 hasConceptScore W1836699491C153911025 @default.
• W1836699491 hasConceptScore W1836699491C166014724 @default.
• W1836699491 hasConceptScore W1836699491C178790620 @default.
• W1836699491 hasConceptScore W1836699491C185581394 @default.
• W1836699491 hasConceptScore W1836699491C185592680 @default.
• W1836699491 hasConceptScore W1836699491C187250156 @default.
• W1836699491 hasConceptScore W1836699491C205260736 @default.
• W1836699491 hasConceptScore W1836699491C2776403692 @default.
• W1836699491 hasConceptScore W1836699491C2779546866 @default.
• W1836699491 hasConceptScore W1836699491C52981337 @default.

• next