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- W1837328148 abstract "pp60c-src is phosphorylated mainly on Ser-17 and Tyr-527 in vivo. In this study, we examined the effect of the phosphorylation of Ser-17 on the properties of pp60c-src by introducing Rous sarcoma virus variants carrying pp60c-src in which Ser-17 had been substituted, into chicken embryo fibroblasts. The Ala-17 substitution in wild-type pp60c-src and pp60c-src carrying Phe-527 caused a two- to threefold elevation in the kinase activity in vitro of these proteins; the former variant resulted in no morphological changes of infected cells, whereas the latter variant transformed chicken embryo fibroblasts. Since the substitution of Tyr-527 per se has been reported to activate pp60c-src, these results suggest that the abolishment of the phosphorylation of Ser-17 does not affect noticeably the properties of pp60c-src in chicken embryo fibroblasts." @default.
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- W1837328148 date "1988-04-01" @default.
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- W1837328148 title "Substitution of Ser-17 of pp60c-src: biological and biochemical characterization in chicken embryo fibroblasts" @default.
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- W1837328148 doi "https://doi.org/10.1128/mcb.8.4.1826-1830.1988" @default.
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