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- W1837630174 abstract "Publisher Summary A principal virtue of polyacrylamide supports in affinity chromatography is low nonspecific adsorption of proteins. In cases where efficient, specific elution is not feasible, this fact becomes of prime importance for achieving a high separation factor. In order to maintain this favorable property, one should avoid loading the support unnecessarily with hydrophobic structures or charged groups. The most likely source of the latter would be carboxylate groups resulting from hydrolysis of the polyacrylamide itself. The derivatization procedures have been designed to keep hydrolysis at a low level. Carboxylate groups can be decreased by treating a gel, such as (X) and presumably other amino forms, with watersoluble carbodiimide (XI) in the presence of 1 M NH 4 C1 at pH 5.4. A similar procedure has been used for this purpose in the case of Sephadex beads and cellulose membranes." @default.
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- W1837630174 date "1974-01-01" @default.
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- W1837630174 title "[3] Covalent linkage of functional groups, ligands, and proteins to polyacrylamide beads" @default.
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- W1837630174 doi "https://doi.org/10.1016/s0076-6879(74)34006-2" @default.
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