FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1837971797> ?p ?o ?g. }

• W1837971797 abstract "Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the CHO-Dhfr expression system. However, CHO cells are known to have a very unstable karyotype. This is due to chromosome rearrangements that can arise from translocations and homologous recombination, especially when cells with the CHO-Dhfr expression system are treated with methotrexate hydrate. The present method used in the industry for testing clones for their long-term stability of recombinant protein production is empirical, and it involves their cultivation over extended periods of time prior to the selection of the most suitable clone for further bioprocess development. The aim of the present study was the identification of marker genes that can predict stable expression of recombinant genes in particular clones early in the development stage.The transcriptome profiles of CHO clones with stable and unstable recombinant protein production were investigated over 10-weeks of cultivation, using a DNA microarray. We identified 14 genes that were differentially expressed between the stable and unstable clones already at 2 weeks from the beginning of the cultivation. Their expression was validated by reverse-transcription quantitative real-time PCR (RT-qPCR). Furthermore, the k-nearest neighbour algorithm approach shows that the combination of the gene expression patterns of only five of these 14 genes is sufficient to predict stable recombinant protein production in clones in the early phases of cell-line development.The exact molecular mechanisms that cause unstable recombinant protein production are not fully understood. However, the expression profiles of some genes in clones with stable and unstable recombinant protein production allow prediction of such instability early in the cell-line development stage. We have thus developed a proof-of-concept for a novel approach to eliminate unstable clones in the CHO-Dhfr expression system, which saves time and labour-intensive work in cell-line development." @default.
• W1837971797 created "2016-06-24" @default.
• W1837971797 creator A5005577105 @default.
• W1837971797 creator A5012297491 @default.
• W1837971797 creator A5019679947 @default.
• W1837971797 creator A5020802355 @default.
• W1837971797 creator A5037127410 @default.
• W1837971797 creator A5038147314 @default.
• W1837971797 creator A5059408217 @default.
• W1837971797 creator A5083172906 @default.
• W1837971797 creator A5084052000 @default.
• W1837971797 creator A5085896670 @default.
• W1837971797 date "2015-10-23" @default.
• W1837971797 modified "2023-10-13" @default.
• W1837971797 title "Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones" @default.
• W1837971797 cites W1546952499 @default.
• W1837971797 cites W1577577364 @default.
• W1837971797 cites W1606589495 @default.
• W1837971797 cites W1969960001 @default.
• W1837971797 cites W1972437720 @default.
• W1837971797 cites W1974887619 @default.
• W1837971797 cites W1990413150 @default.
• W1837971797 cites W1993363553 @default.
• W1837971797 cites W1998725815 @default.
• W1837971797 cites W2012175027 @default.
• W1837971797 cites W2012332159 @default.
• W1837971797 cites W2013766823 @default.
• W1837971797 cites W2015298622 @default.
• W1837971797 cites W2019508778 @default.
• W1837971797 cites W2025643953 @default.
• W1837971797 cites W2027783748 @default.
• W1837971797 cites W2027852704 @default.
• W1837971797 cites W2028986416 @default.
• W1837971797 cites W2033032210 @default.
• W1837971797 cites W2036014270 @default.
• W1837971797 cites W2043911972 @default.
• W1837971797 cites W2046147149 @default.
• W1837971797 cites W2048666997 @default.
• W1837971797 cites W2048941695 @default.
• W1837971797 cites W2054584654 @default.
• W1837971797 cites W2055096917 @default.
• W1837971797 cites W2056983875 @default.
• W1837971797 cites W2062989416 @default.
• W1837971797 cites W2065077716 @default.
• W1837971797 cites W2071702521 @default.
• W1837971797 cites W2075808870 @default.
• W1837971797 cites W2078098552 @default.
• W1837971797 cites W2085754069 @default.
• W1837971797 cites W2087086600 @default.
• W1837971797 cites W2088165670 @default.
• W1837971797 cites W2088557075 @default.
• W1837971797 cites W2091713101 @default.
• W1837971797 cites W2092211068 @default.
• W1837971797 cites W2093065228 @default.
• W1837971797 cites W2093132278 @default.
• W1837971797 cites W2100989340 @default.
• W1837971797 cites W2106341962 @default.
• W1837971797 cites W2108244474 @default.
• W1837971797 cites W2108408221 @default.
• W1837971797 cites W2109972881 @default.
• W1837971797 cites W2115124797 @default.
• W1837971797 cites W2120679753 @default.
• W1837971797 cites W2120865735 @default.
• W1837971797 cites W2121074166 @default.
• W1837971797 cites W2124664751 @default.
• W1837971797 cites W2133513884 @default.
• W1837971797 cites W2141413204 @default.
• W1837971797 cites W2143346248 @default.
• W1837971797 cites W2155218352 @default.
• W1837971797 cites W2155421287 @default.
• W1837971797 cites W2163107106 @default.
• W1837971797 cites W2169976220 @default.
• W1837971797 cites W2172223940 @default.
• W1837971797 cites W257693959 @default.
• W1837971797 cites W4235611253 @default.
• W1837971797 cites W4244544159 @default.
• W1837971797 cites W82569036 @default.
• W1837971797 doi "https://doi.org/10.1186/s12896-015-0218-9" @default.
• W1837971797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4812793" @default.
• W1837971797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26499110" @default.
• W1837971797 hasPublicationYear "2015" @default.
• W1837971797 type Work @default.
• W1837971797 sameAs 1837971797 @default.
• W1837971797 citedByCount "9" @default.
• W1837971797 countsByYear W18379717972016 @default.
• W1837971797 countsByYear W18379717972017 @default.
• W1837971797 countsByYear W18379717972019 @default.
• W1837971797 countsByYear W18379717972021 @default.
• W1837971797 countsByYear W18379717972023 @default.
• W1837971797 crossrefType "journal-article" @default.
• W1837971797 hasAuthorship W1837971797A5005577105 @default.
• W1837971797 hasAuthorship W1837971797A5012297491 @default.
• W1837971797 hasAuthorship W1837971797A5019679947 @default.
• W1837971797 hasAuthorship W1837971797A5020802355 @default.
• W1837971797 hasAuthorship W1837971797A5037127410 @default.
• W1837971797 hasAuthorship W1837971797A5038147314 @default.
• W1837971797 hasAuthorship W1837971797A5059408217 @default.
• W1837971797 hasAuthorship W1837971797A5083172906 @default.
• W1837971797 hasAuthorship W1837971797A5084052000 @default.
• W1837971797 hasAuthorship W1837971797A5085896670 @default.

• next