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• W1838113806 abstract "Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P. aeruginosa strains that are widespread in patients in Kurdistan region of Iraq, 165 clinical samples were collected from wounds, burn, ear infection and urinary tract infection taken from general hospitals of different areas of the region. Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, were used in this study. The results show that, the selective medium (cetrimide agar) at 42˚C aerobically had highest recovery in the isolation of P. aeruginosa strains, they were produced greenish-yellow or blue pigment colonies, catalase and oxidase was positive whereas negative for methyl red, Voges Proskauer and indole.; however, some of these methods are time consuming and may not be very accurate whereas API 20E is rapid method which performs at least 20 different biochemical tests at once, however it proved difficult to obtain additional information concerning the relationship between these strains. To study the susceptibility of tested strain to different antibiotics using disk diffusion the result show that all the strains of P. aeruginosa were resistance to Cefotaxime, Chloramphenicol, Clindomycin, Erythromycin, Gentamycin, Nitrofurantoin and Pincillin, and susceptible to Ciprofloxacin, Imipenin, Lincomycin, Piperacillin and Tobramycin, while the strains showed differs susceptibility for Amikacin, Nalidixic acid, Rifampicin, Tetracycline and Trimethoprim.. In attempting to identification of P. aeruginosa strains at the DNA level, Polymerase chain reaction (PCR) is used based on specific primer for 16S rRNA and OPRL gene , the results showed that PCR has found to be rapid and more sensitive and specific in identification of P. aeruginosa, however ,combination of more than one specific marker is recommended." @default.
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• W1838113806 date "2012-09-17" @default.
• W1838113806 modified "2023-09-27" @default.
• W1838113806 title "Molecular identification of Pseudomonas aeruginosa isolated from Hospitals in Kurdistan region" @default.
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