FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1838698933> ?p ?o ?g. }

• W1838698933 endingPage "15" @default.
• W1838698933 startingPage "15" @default.
• W1838698933 abstract "Beta2-adrenergic receptors (beta2AR) play important regulatory roles in a variety of cells and organ systems and are important therapeutic targets in the treatment of airway and cardiovascular disease. Prolonged use of beta-agonists results in tolerance secondary to receptor down-regulation resulting in reduced therapeutic efficiency. The purpose of this work is to evaluate the signaling capabilities of the beta2AR expressed by a recombinant adeno-associated viral (AAV) vector that also included an enhanced green fluorescent protein (EGFP) gene (AAV-beta2AR/EGFP).By epifluorescence microscopy, approximately 40% of infected HEK 293 cells demonstrated EGFP expression. beta2AR density measured with [3H]dihydroalprenolol ([3H]DHA) increased either 13- or 77-fold in infected cells compared to mock infected controls depending on the culture conditions used. The [3H]DHA binding was to a single receptor population with a dissociation constant of 0.42 nM, as would be expected for wild-type beta2AR. Agonist competition assays with [3H]DHA showed the following rank order of potency: isoproterenol>epinephrine> norepinephrine, consistent with beta2AR interaction. Isoproterenol-stimulated cyclic AMP levels were 5-fold higher in infected cells compared to controls (314 +/- 43 vs. 63.4 +/- 9.6 nmol/dish; n = 3). Receptor trafficking demonstrated surface expression of beta2AR with vehicle treatment and internalization following isoproterenol treatment.We conclude that HEK 293 cells infected with AAV-beta2AR/EGFP effectively express beta2AR and that increased expression of these receptors results in enhanced beta2AR signaling. This method of gene transfer may provide an important means to enhance function in in vivo systems." @default.
• W1838698933 created "2016-06-24" @default.
• W1838698933 creator A5027978088 @default.
• W1838698933 creator A5056459231 @default.
• W1838698933 creator A5064243731 @default.
• W1838698933 creator A5069262812 @default.
• W1838698933 creator A5082318531 @default.
• W1838698933 creator A5085949410 @default.
• W1838698933 date "2003-01-01" @default.
• W1838698933 modified "2023-09-27" @default.
• W1838698933 cites W1483189363 @default.
• W1838698933 cites W1507084693 @default.
• W1838698933 cites W1571519589 @default.
• W1838698933 cites W1889620018 @default.
• W1838698933 cites W1978195147 @default.
• W1838698933 cites W1985164864 @default.
• W1838698933 cites W1990951259 @default.
• W1838698933 cites W2003686368 @default.
• W1838698933 cites W2007639666 @default.
• W1838698933 cites W2021711796 @default.
• W1838698933 cites W2024175375 @default.
• W1838698933 cites W2043698693 @default.
• W1838698933 cites W2043813981 @default.
• W1838698933 cites W2059141314 @default.
• W1838698933 cites W2073457809 @default.
• W1838698933 cites W2074631079 @default.
• W1838698933 cites W2076540700 @default.
• W1838698933 cites W2090691719 @default.
• W1838698933 cites W2101197866 @default.
• W1838698933 cites W2113378908 @default.
• W1838698933 cites W2128635872 @default.
• W1838698933 cites W2157572122 @default.
• W1838698933 cites W2159230973 @default.
• W1838698933 cites W2172060077 @default.
• W1838698933 cites W2327324759 @default.
• W1838698933 cites W2341351199 @default.
• W1838698933 cites W2412428830 @default.
• W1838698933 cites W45551491 @default.
• W1838698933 doi "https://doi.org/10.1186/1471-2210-3-15" @default.
• W1838698933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/317295" @default.
• W1838698933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14656380" @default.
• W1838698933 hasPublicationYear "2003" @default.
• W1838698933 type Work @default.
• W1838698933 sameAs 1838698933 @default.
• W1838698933 citedByCount "11" @default.
• W1838698933 countsByYear W18386989332012 @default.
• W1838698933 countsByYear W18386989332017 @default.
• W1838698933 countsByYear W18386989332019 @default.
• W1838698933 crossrefType "journal-article" @default.
• W1838698933 hasAuthorship W1838698933A5027978088 @default.
• W1838698933 hasAuthorship W1838698933A5056459231 @default.
• W1838698933 hasAuthorship W1838698933A5064243731 @default.
• W1838698933 hasAuthorship W1838698933A5069262812 @default.
• W1838698933 hasAuthorship W1838698933A5082318531 @default.
• W1838698933 hasAuthorship W1838698933A5085949410 @default.
• W1838698933 hasBestOaLocation W18386989331 @default.
• W1838698933 hasConcept C104317684 @default.
• W1838698933 hasConcept C126322002 @default.
• W1838698933 hasConcept C134018914 @default.
• W1838698933 hasConcept C139770010 @default.
• W1838698933 hasConcept C142613039 @default.
• W1838698933 hasConcept C170493617 @default.
• W1838698933 hasConcept C174510640 @default.
• W1838698933 hasConcept C185592680 @default.
• W1838698933 hasConcept C2778938600 @default.
• W1838698933 hasConcept C55493867 @default.
• W1838698933 hasConcept C71924100 @default.
• W1838698933 hasConcept C86803240 @default.
• W1838698933 hasConcept C95444343 @default.
• W1838698933 hasConcept C98274493 @default.
• W1838698933 hasConceptScore W1838698933C104317684 @default.
• W1838698933 hasConceptScore W1838698933C126322002 @default.
• W1838698933 hasConceptScore W1838698933C134018914 @default.
• W1838698933 hasConceptScore W1838698933C139770010 @default.
• W1838698933 hasConceptScore W1838698933C142613039 @default.
• W1838698933 hasConceptScore W1838698933C170493617 @default.
• W1838698933 hasConceptScore W1838698933C174510640 @default.
• W1838698933 hasConceptScore W1838698933C185592680 @default.
• W1838698933 hasConceptScore W1838698933C2778938600 @default.
• W1838698933 hasConceptScore W1838698933C55493867 @default.
• W1838698933 hasConceptScore W1838698933C71924100 @default.
• W1838698933 hasConceptScore W1838698933C86803240 @default.
• W1838698933 hasConceptScore W1838698933C95444343 @default.
• W1838698933 hasConceptScore W1838698933C98274493 @default.
• W1838698933 hasIssue "1" @default.
• W1838698933 hasLocation W18386989331 @default.
• W1838698933 hasLocation W18386989332 @default.
• W1838698933 hasLocation W18386989333 @default.
• W1838698933 hasLocation W18386989334 @default.
• W1838698933 hasOpenAccess W1838698933 @default.
• W1838698933 hasPrimaryLocation W18386989331 @default.
• W1838698933 hasRelatedWork W1988087328 @default.
• W1838698933 hasRelatedWork W2027913950 @default.
• W1838698933 hasRelatedWork W2034583712 @default.
• W1838698933 hasRelatedWork W2055958222 @default.
• W1838698933 hasRelatedWork W2063599511 @default.
• W1838698933 hasRelatedWork W2063619316 @default.
• W1838698933 hasRelatedWork W2101411396 @default.

• next