Matches in SemOpenAlex for { <https://semopenalex.org/work/W1838776087> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1838776087 endingPage "303" @default.
- W1838776087 startingPage "295" @default.
- W1838776087 abstract "Due to increases in greenhouse gas emissions, it is necessary to explore renewable sources of energy. Interesting alternatives are biofuels derived from microalgae. One challenge is the development of a detailed microalgae database compiling species identifications and characterizations that would facilitate microalgae selection for biomass production. Mass spectrometric (MS) analysis using a matrix-assisted laser desorption/ionization (MALDI) source is an advanced technique that enables advancement in this biological area. In this work a MALDI time-of-flight (TOF)MS method for the rapid identification of proteins in whole cells of selected microalgae species was studied. Furthermore, the efficiency of different matrix and solvent systems was tested. MS analyses were performed using an UltrafleXtreme MALDI-TOF mass spectrometer operating in linear positive ion mode.Mass spectra were acquired in a mass range from 4000 to 20,000 Da with ions generated from Smartbeam laser irradiation using a frequency of 2000 Hz, a PIE 100 ns and a lens 7 kV. The voltage was 25 kV for the first ion source and 23 kV for the second. Each spectrum was generated by averaging of 10,000 laser shots and the laser irradiance was set at 95-100%.Similar mass spectra were obtained for all matrices (SA, HCCA, DHB and sDHB); however, the use of the sDHB matrix resulted in spectrum profiles with a greater amount number of proteins, a better signal/noise (S/N) ratio and higher intensities for the majority of microalgae analyzed. Trifluoroacetic acid (TFA) content was also studied and the best results in terms of S/N ratio, number of proteins and signal intensities were obtained with 0.1% TFA in the matrix solvent. The addition of isopropanol did not produce improvement in the quality of spectrum profiles.Therefore, the optimal matrix for the analysis of protein from intact microalgae cells is sDHB with TA50 as the matrix solvent and without isopropanol. These conditions allow the acquisition of high quality spectrum profiles." @default.
- W1838776087 created "2016-06-24" @default.
- W1838776087 creator A5009794660 @default.
- W1838776087 creator A5051117227 @default.
- W1838776087 creator A5075590407 @default.
- W1838776087 creator A5081132762 @default.
- W1838776087 date "2014-12-29" @default.
- W1838776087 modified "2023-10-16" @default.
- W1838776087 title "Comparative study of different matrix/solvent systems for the analysis of crude lyophilized microalgal preparations using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry" @default.
- W1838776087 cites W1904826041 @default.
- W1838776087 cites W1965757778 @default.
- W1838776087 cites W1973080027 @default.
- W1838776087 cites W2012380814 @default.
- W1838776087 cites W2046454389 @default.
- W1838776087 cites W2059349849 @default.
- W1838776087 cites W2076209159 @default.
- W1838776087 cites W2121627374 @default.
- W1838776087 cites W2158136248 @default.
- W1838776087 cites W2172112723 @default.
- W1838776087 doi "https://doi.org/10.1002/rcm.7110" @default.
- W1838776087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26411627" @default.
- W1838776087 hasPublicationYear "2014" @default.
- W1838776087 type Work @default.
- W1838776087 sameAs 1838776087 @default.
- W1838776087 citedByCount "15" @default.
- W1838776087 countsByYear W18387760872015 @default.
- W1838776087 countsByYear W18387760872016 @default.
- W1838776087 countsByYear W18387760872017 @default.
- W1838776087 countsByYear W18387760872018 @default.
- W1838776087 countsByYear W18387760872020 @default.
- W1838776087 countsByYear W18387760872021 @default.
- W1838776087 countsByYear W18387760872022 @default.
- W1838776087 countsByYear W18387760872023 @default.
- W1838776087 crossrefType "journal-article" @default.
- W1838776087 hasAuthorship W1838776087A5009794660 @default.
- W1838776087 hasAuthorship W1838776087A5051117227 @default.
- W1838776087 hasAuthorship W1838776087A5075590407 @default.
- W1838776087 hasAuthorship W1838776087A5081132762 @default.
- W1838776087 hasConcept C106487976 @default.
- W1838776087 hasConcept C113196181 @default.
- W1838776087 hasConcept C120665830 @default.
- W1838776087 hasConcept C121332964 @default.
- W1838776087 hasConcept C145148216 @default.
- W1838776087 hasConcept C150394285 @default.
- W1838776087 hasConcept C162356407 @default.
- W1838776087 hasConcept C162711632 @default.
- W1838776087 hasConcept C16291436 @default.
- W1838776087 hasConcept C178790620 @default.
- W1838776087 hasConcept C185592680 @default.
- W1838776087 hasConcept C188224857 @default.
- W1838776087 hasConcept C198291218 @default.
- W1838776087 hasConcept C40325409 @default.
- W1838776087 hasConcept C43617362 @default.
- W1838776087 hasConcept C520434653 @default.
- W1838776087 hasConcept C75280812 @default.
- W1838776087 hasConceptScore W1838776087C106487976 @default.
- W1838776087 hasConceptScore W1838776087C113196181 @default.
- W1838776087 hasConceptScore W1838776087C120665830 @default.
- W1838776087 hasConceptScore W1838776087C121332964 @default.
- W1838776087 hasConceptScore W1838776087C145148216 @default.
- W1838776087 hasConceptScore W1838776087C150394285 @default.
- W1838776087 hasConceptScore W1838776087C162356407 @default.
- W1838776087 hasConceptScore W1838776087C162711632 @default.
- W1838776087 hasConceptScore W1838776087C16291436 @default.
- W1838776087 hasConceptScore W1838776087C178790620 @default.
- W1838776087 hasConceptScore W1838776087C185592680 @default.
- W1838776087 hasConceptScore W1838776087C188224857 @default.
- W1838776087 hasConceptScore W1838776087C198291218 @default.
- W1838776087 hasConceptScore W1838776087C40325409 @default.
- W1838776087 hasConceptScore W1838776087C43617362 @default.
- W1838776087 hasConceptScore W1838776087C520434653 @default.
- W1838776087 hasConceptScore W1838776087C75280812 @default.
- W1838776087 hasIssue "3" @default.
- W1838776087 hasLocation W18387760871 @default.
- W1838776087 hasLocation W18387760872 @default.
- W1838776087 hasOpenAccess W1838776087 @default.
- W1838776087 hasPrimaryLocation W18387760871 @default.
- W1838776087 hasRelatedWork W1973847141 @default.
- W1838776087 hasRelatedWork W2003619873 @default.
- W1838776087 hasRelatedWork W2007500700 @default.
- W1838776087 hasRelatedWork W2065486654 @default.
- W1838776087 hasRelatedWork W2067018545 @default.
- W1838776087 hasRelatedWork W2072153628 @default.
- W1838776087 hasRelatedWork W2115089515 @default.
- W1838776087 hasRelatedWork W2154308360 @default.
- W1838776087 hasRelatedWork W2320691067 @default.
- W1838776087 hasRelatedWork W2380378082 @default.
- W1838776087 hasVolume "29" @default.
- W1838776087 isParatext "false" @default.
- W1838776087 isRetracted "false" @default.
- W1838776087 magId "1838776087" @default.
- W1838776087 workType "article" @default.