FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1838779145> ?p ?o ?g. }

• W1838779145 abstract "Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function at low temperatures. Recombinant production presents a significant barrier to their exploitation for commercial applications in industry.As part of an enzyme discovery project we have investigated the utility of a cold-shock inducible promoter for low-temperature expression of five diverse genes derived from the metagenomes of marine Arctic sediments. After evaluation of their production, we further optimized for soluble production by building a vector suite from which the environmental genes could be expressed as fusions with solubility tags.We found that the low-temperature optimized system produced high expression levels for all putatively cold-active proteins, as well as reducing host toxicity for several candidates. As a proof of concept, activity assays with one of the candidates, a putative chitinase, showed that functional protein was obtained using the low-temperature optimized vector suite.We conclude that a cold-shock inducible system is advantageous for the heterologous expression of psychrophilic proteins, and may also be useful for expression of toxic mesophilic and thermophilic proteins where properties of the proteins are deleterious to the host cell growth." @default.
• W1838779145 created "2016-06-24" @default.
• W1838779145 creator A5034008820 @default.
• W1838779145 creator A5041548835 @default.
• W1838779145 date "2015-08-19" @default.
• W1838779145 modified "2023-10-16" @default.
• W1838779145 title "Cold shock induction of recombinant Arctic environmental genes" @default.
• W1838779145 cites W1515935760 @default.
• W1838779145 cites W1533135956 @default.
• W1838779145 cites W1536886974 @default.
• W1838779145 cites W1539744755 @default.
• W1838779145 cites W1550045826 @default.
• W1838779145 cites W1585692793 @default.
• W1838779145 cites W1965640337 @default.
• W1838779145 cites W1969175129 @default.
• W1838779145 cites W1970386271 @default.
• W1838779145 cites W1978327058 @default.
• W1838779145 cites W1979861170 @default.
• W1838779145 cites W1987534021 @default.
• W1838779145 cites W1988479656 @default.
• W1838779145 cites W1988583600 @default.
• W1838779145 cites W1992100167 @default.
• W1838779145 cites W1992221689 @default.
• W1838779145 cites W1996822335 @default.
• W1838779145 cites W2001638063 @default.
• W1838779145 cites W2002142911 @default.
• W1838779145 cites W2004583149 @default.
• W1838779145 cites W2007527881 @default.
• W1838779145 cites W2012604733 @default.
• W1838779145 cites W2012643575 @default.
• W1838779145 cites W2013818525 @default.
• W1838779145 cites W2015119618 @default.
• W1838779145 cites W2016774313 @default.
• W1838779145 cites W2020719323 @default.
• W1838779145 cites W2026497552 @default.
• W1838779145 cites W2027122403 @default.
• W1838779145 cites W2028994826 @default.
• W1838779145 cites W2035260123 @default.
• W1838779145 cites W2039707307 @default.
• W1838779145 cites W2040606884 @default.
• W1838779145 cites W2043335299 @default.
• W1838779145 cites W2045242156 @default.
• W1838779145 cites W2048673910 @default.
• W1838779145 cites W2050790746 @default.
• W1838779145 cites W2050976380 @default.
• W1838779145 cites W2055043387 @default.
• W1838779145 cites W2056009722 @default.
• W1838779145 cites W2060297307 @default.
• W1838779145 cites W2060810338 @default.
• W1838779145 cites W2065366746 @default.
• W1838779145 cites W2067110039 @default.
• W1838779145 cites W2069848278 @default.
• W1838779145 cites W2075668933 @default.
• W1838779145 cites W2076117093 @default.
• W1838779145 cites W2080434522 @default.
• W1838779145 cites W2081859323 @default.
• W1838779145 cites W2083899848 @default.
• W1838779145 cites W2092471412 @default.
• W1838779145 cites W2092827855 @default.
• W1838779145 cites W2094201044 @default.
• W1838779145 cites W2097152121 @default.
• W1838779145 cites W2098303032 @default.
• W1838779145 cites W2098817626 @default.
• W1838779145 cites W2099200059 @default.
• W1838779145 cites W2100234079 @default.
• W1838779145 cites W2103172172 @default.
• W1838779145 cites W2104557649 @default.
• W1838779145 cites W2104613138 @default.
• W1838779145 cites W2108072410 @default.
• W1838779145 cites W2108859348 @default.
• W1838779145 cites W2109807697 @default.
• W1838779145 cites W2109925162 @default.
• W1838779145 cites W2120641909 @default.
• W1838779145 cites W2122500226 @default.
• W1838779145 cites W2122559203 @default.
• W1838779145 cites W2128114769 @default.
• W1838779145 cites W2132612000 @default.
• W1838779145 cites W2132656109 @default.
• W1838779145 cites W2141000676 @default.
• W1838779145 cites W2141884970 @default.
• W1838779145 cites W2145097156 @default.
• W1838779145 cites W2147526198 @default.
• W1838779145 cites W2149367664 @default.
• W1838779145 cites W2152301430 @default.
• W1838779145 cites W2156377681 @default.
• W1838779145 cites W2162875202 @default.
• W1838779145 cites W2165600016 @default.
• W1838779145 cites W2167171219 @default.
• W1838779145 cites W2168383728 @default.
• W1838779145 cites W2170667639 @default.
• W1838779145 cites W2622160543 @default.
• W1838779145 cites W188593863 @default.
• W1838779145 doi "https://doi.org/10.1186/s12896-015-0185-1" @default.
• W1838779145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4544801" @default.
• W1838779145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26286037" @default.
• W1838779145 hasPublicationYear "2015" @default.
• W1838779145 type Work @default.
• W1838779145 sameAs 1838779145 @default.
• W1838779145 citedByCount "13" @default.
• W1838779145 countsByYear W18387791452016 @default.

• next