Matches in SemOpenAlex for { <https://semopenalex.org/work/W1838780003> ?p ?o ?g. }
- W1838780003 endingPage "2547" @default.
- W1838780003 startingPage "2532" @default.
- W1838780003 abstract "Focal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1–LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 that are binding sites for focal adhesion kinase (FAK) and other proteins. Phage display selections against peptides were used to generate sABs recognizing each LD motif. In the obtained X-ray crystal structures of the LD-sAB complexes, the LD motifs are helical and bind sABs through a hydrophobic side, similarly as in the structures with natural paxillin partners. The sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin in focal adhesions in cells. They were also used as selective inhibitors to effectively compete with focal adhesion targeting domain of FAK for the binding to LD2 and LD4. The sABs are tools for investigation of paxillin LD binding “platforms” and are capable of inhibiting paxillin interactions, thereby useful as potential therapeutics in the future." @default.
- W1838780003 created "2016-06-24" @default.
- W1838780003 creator A5003826371 @default.
- W1838780003 creator A5048017462 @default.
- W1838780003 creator A5060281780 @default.
- W1838780003 creator A5067335481 @default.
- W1838780003 date "2015-07-01" @default.
- W1838780003 modified "2023-10-10" @default.
- W1838780003 title "Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin" @default.
- W1838780003 cites W1488252042 @default.
- W1838780003 cites W1539796472 @default.
- W1838780003 cites W1967859438 @default.
- W1838780003 cites W1973334306 @default.
- W1838780003 cites W1975723984 @default.
- W1838780003 cites W1977585994 @default.
- W1838780003 cites W1983335488 @default.
- W1838780003 cites W1988664861 @default.
- W1838780003 cites W1990031371 @default.
- W1838780003 cites W2010499485 @default.
- W1838780003 cites W2011276624 @default.
- W1838780003 cites W2021064989 @default.
- W1838780003 cites W2036145275 @default.
- W1838780003 cites W2036183783 @default.
- W1838780003 cites W2052639416 @default.
- W1838780003 cites W2058170261 @default.
- W1838780003 cites W2061708016 @default.
- W1838780003 cites W2066367871 @default.
- W1838780003 cites W2067427407 @default.
- W1838780003 cites W2072183005 @default.
- W1838780003 cites W2072583244 @default.
- W1838780003 cites W2092488180 @default.
- W1838780003 cites W2094550032 @default.
- W1838780003 cites W2102801515 @default.
- W1838780003 cites W2107552761 @default.
- W1838780003 cites W2108921801 @default.
- W1838780003 cites W2109236773 @default.
- W1838780003 cites W2112093320 @default.
- W1838780003 cites W2112418598 @default.
- W1838780003 cites W2112630221 @default.
- W1838780003 cites W2116312858 @default.
- W1838780003 cites W2120346794 @default.
- W1838780003 cites W2122875377 @default.
- W1838780003 cites W2124026197 @default.
- W1838780003 cites W2129060396 @default.
- W1838780003 cites W2140506935 @default.
- W1838780003 cites W2156451315 @default.
- W1838780003 cites W2159211495 @default.
- W1838780003 cites W2163341755 @default.
- W1838780003 cites W2167956058 @default.
- W1838780003 cites W2168918180 @default.
- W1838780003 cites W4248872320 @default.
- W1838780003 doi "https://doi.org/10.1016/j.jmb.2015.06.004" @default.
- W1838780003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4521624" @default.
- W1838780003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26087144" @default.
- W1838780003 hasPublicationYear "2015" @default.
- W1838780003 type Work @default.
- W1838780003 sameAs 1838780003 @default.
- W1838780003 citedByCount "9" @default.
- W1838780003 countsByYear W18387800032017 @default.
- W1838780003 countsByYear W18387800032020 @default.
- W1838780003 countsByYear W18387800032021 @default.
- W1838780003 countsByYear W18387800032022 @default.
- W1838780003 countsByYear W18387800032023 @default.
- W1838780003 crossrefType "journal-article" @default.
- W1838780003 hasAuthorship W1838780003A5003826371 @default.
- W1838780003 hasAuthorship W1838780003A5048017462 @default.
- W1838780003 hasAuthorship W1838780003A5060281780 @default.
- W1838780003 hasAuthorship W1838780003A5067335481 @default.
- W1838780003 hasBestOaLocation W18387800031 @default.
- W1838780003 hasConcept C104317684 @default.
- W1838780003 hasConcept C108391706 @default.
- W1838780003 hasConcept C116581196 @default.
- W1838780003 hasConcept C11960822 @default.
- W1838780003 hasConcept C142669718 @default.
- W1838780003 hasConcept C1491633281 @default.
- W1838780003 hasConcept C151166631 @default.
- W1838780003 hasConcept C153911025 @default.
- W1838780003 hasConcept C170493617 @default.
- W1838780003 hasConcept C185592680 @default.
- W1838780003 hasConcept C186268636 @default.
- W1838780003 hasConcept C195687474 @default.
- W1838780003 hasConcept C24284526 @default.
- W1838780003 hasConcept C2779281246 @default.
- W1838780003 hasConcept C2993400109 @default.
- W1838780003 hasConcept C5432534 @default.
- W1838780003 hasConcept C55493867 @default.
- W1838780003 hasConcept C86339819 @default.
- W1838780003 hasConcept C86803240 @default.
- W1838780003 hasConcept C90934575 @default.
- W1838780003 hasConcept C95444343 @default.
- W1838780003 hasConcept C97029542 @default.
- W1838780003 hasConceptScore W1838780003C104317684 @default.
- W1838780003 hasConceptScore W1838780003C108391706 @default.
- W1838780003 hasConceptScore W1838780003C116581196 @default.
- W1838780003 hasConceptScore W1838780003C11960822 @default.
- W1838780003 hasConceptScore W1838780003C142669718 @default.
- W1838780003 hasConceptScore W1838780003C1491633281 @default.
- W1838780003 hasConceptScore W1838780003C151166631 @default.