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- W183907315 abstract "Soil microbes especially actinomycetes are a main source of the production of bioactive metabolites through its secondary metabolisms. Therefore, soils are collected atthe rain forest of Danum valley during the field trip to Danum Valley. The collected soil samples are used for the isolation and purification of actinomycetes using humic acidvitamin agar and oatmeal agar. Purified actinomycetes are fermented by using mannitolpeptone medium for the production of secondary metabolites. Besides that, one assign strain of actinomycetes, H7372, which is isolated by Cheah, is fermented using the KF-7L fermentor. Fermentation of the actinomycetes are ended after 120 hours and harvested byadding acetone at the proportion of 1:1 (v/v). Acetone extract obtained from the fermentation is used for the screening for bioactive metabolites against mammalian GSK-3I² and Ras/Raf-1 protein interaction. Besides that, Myrmecodia tuberosa, which is a medicinal plant that found in the forest of Sabah is also tested. This medicinal plant is extracted using two different extraction methods, which are solid-liquid extraction system and liquid-liquid extraction system. From the liquid-liquid extraction, two different layers, which are the methanol aqueous and chloroform layer are used in the screening againstmammalian GSK -3I² and also Ras/Raf-1 protein interaction. In the screening of bioactive metabolites against mammalian GSK-3I², H10075 and H10079, which is the gsk-3 nullmutant transform with pKT10-GSK3I² and Yep24-MCK1 respectively. Yeast two-hybrid screening system is used in the screening for inhibitors against Ras/Raf-1 proteininteraction. Total of 7 of 94 new isolates of Danum Valley, which are H11695, H11722, Hl1821, H11853, Hl1855, H11856 and H11857, showing inhibition in the screening against GSK-3I² While, 9 of 94 isolates, which are H11822, H11855, H11856, H118S7, H11858, H11861, H11867, H11878 and H11879 showing inhibition against Ras/Raf-1. The medicinal plants extract did not show inhibition effects against both of screeningsystem. The inhibitor can be distinguished into two types that are potential and toxic inhibitor. Potential inhibitors are the inhibitor that will inhibit the abnormal cells whilepermitting the growth of normal cells; meanwhile toxic inhibitors are inhibitor that will inhibit both of the normal and abnormal cell growth in the RaslRaf-1 and GSK-3 pathway." @default.
- W183907315 created "2016-06-24" @default.
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- W183907315 date "2005-01-01" @default.
- W183907315 modified "2023-09-27" @default.
- W183907315 title "Screening and purification for inhibitors of GSK3 and RAS/RAF from actinomycetes and myrmecodia tuberosa" @default.
- W183907315 hasPublicationYear "2005" @default.
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