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- W1839110188 abstract "The microflora of two acidic environments was investigated using analysis of 16SrDNA amplified by the polymerase chain reaction (PCR) from environmental DNA.These environments had different chemical characteristics from most of the acidicenvironments studied by others. The first sample site, a coal spoil (Birch Coppice,Warwickshire), might have been expected to produce niches enriched in humic matter.The second, comprising geothermal vents on the Island of Vulcano, was unusual fornatural acidic environments since it was saline. Three vent regions of differenttemperatures (30°C, 45°C and 80°C) were examined.Prior to the 16S rDNA analysis of the sites, a brief investigation into selection of asuitable method of DNA extraction was carried out. A bead-beating method and achemical lysis/freeze-thaw method were compared. With regard to clone types found viaeach method, there was little qualitative difference. DNA was extracted from the twosites and 16S rRNA genes were amplified by PCR. PCR products were ligated andcompetent E. coli cells were transformed to produce clone libraries. Restriction fragmentlength polymorphisms (RFLPs) were examined and representatives of each RFLP typewere sequenced and analysed with reference to RNA gene sequence data bases.The coal spoil clone library was dominated by sequences related to those fromuncultured actinobacteria, particularly those found previously in peat bogs and varioussoils. Representatives of some well-known acidophiles were also found (e.g.Leptospirillum species). The clone bank from the saline, geothermal site DNA comprisedsequences from acidophiles capable of growth at the respective temperatures of differentsamples. The lowest temperature samples produced sequences from a novelAcidithiobacillus species and also indicated a novel species probably related toThiobacillus prosperus (which was isolated previously from Vulcano). A hightemperature sample gave sequences from archaeal acidophiles, Acidianus brierleyi and,previously isolated from Vulcano, Acidianus infernus and Thermoplasma volcanium.Where the clone banks revealed the presence of novel organisms, attempts weremade to isolate and characterise them. The novel actinobacteria did not appear to grow inlaboratory enrichment cultures. The novel Acidithiobacillus species and two novelThiobacillus prosperus-like species were characterised." @default.
- W1839110188 created "2016-06-24" @default.
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- W1839110188 date "2001-09-01" @default.
- W1839110188 modified "2023-09-24" @default.
- W1839110188 title "The microbial ecology of acidic environments" @default.
- W1839110188 hasPublicationYear "2001" @default.
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