Matches in SemOpenAlex for { <https://semopenalex.org/work/W1839466403> ?p ?o ?g. }
- W1839466403 endingPage "699" @default.
- W1839466403 startingPage "690" @default.
- W1839466403 abstract "In this paper we report on the development of dynamically controlled three-dimensional (3D) micropatterned cellular co-cultures within photocurable and chemically degradable hydrogels. Specifically, we generated dynamic co-cultures of micropatterned murine embryonic stem (mES) cells with human hepatocellular carcinoma (HepG2) cells within 3D hydrogels. HepG2 cells were used due to their ability to direct the differentiation of mES cells through secreted paracrine factors. To generate dynamic co-cultures, mES cells were first encapsulated within micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. These micropatterned cell-laden PEG hydrogels were subsequently surrounded by calcium alginate (Ca-Alg) hydrogels containing HepG2 cells. After 4 days, the co-culture step was halted by exposing the system to sodium citrate solution, which removed the alginate gels and the encapsulated HepG2 cells. The encapsulated mES cells were then maintained in the resulting cultures for 16 days and cardiac differentiation was analysed. We observed that the mES cells that were exposed to HepG2 cells in the co-cultures generated cells with higher expression of cardiac genes and proteins, as well as increased spontaneous beating. Due to its ability to control the 3D microenvironment of cells in a spatially and temporally regulated manner, the method presented in this study is useful for a range of cell-culture applications related to tissue engineering and regenerative medicine. Copyright © 2013 John Wiley & Sons, Ltd." @default.
- W1839466403 created "2016-06-24" @default.
- W1839466403 creator A5033056518 @default.
- W1839466403 creator A5054766668 @default.
- W1839466403 creator A5058060254 @default.
- W1839466403 creator A5072121602 @default.
- W1839466403 creator A5086619153 @default.
- W1839466403 creator A5090778413 @default.
- W1839466403 date "2013-10-30" @default.
- W1839466403 modified "2023-09-23" @default.
- W1839466403 title "Dynamic three-dimensional micropatterned cell co-cultures within photocurable and chemically degradable hydrogels" @default.
- W1839466403 cites W1503084282 @default.
- W1839466403 cites W1575499472 @default.
- W1839466403 cites W1832267083 @default.
- W1839466403 cites W1953604676 @default.
- W1839466403 cites W1964527179 @default.
- W1839466403 cites W1966090583 @default.
- W1839466403 cites W1973318486 @default.
- W1839466403 cites W1973780146 @default.
- W1839466403 cites W1973997155 @default.
- W1839466403 cites W1974452230 @default.
- W1839466403 cites W1974826600 @default.
- W1839466403 cites W1975013625 @default.
- W1839466403 cites W1976842528 @default.
- W1839466403 cites W1978918367 @default.
- W1839466403 cites W1979263756 @default.
- W1839466403 cites W1982798313 @default.
- W1839466403 cites W1986158400 @default.
- W1839466403 cites W1987778104 @default.
- W1839466403 cites W1988152718 @default.
- W1839466403 cites W1990645229 @default.
- W1839466403 cites W1991111225 @default.
- W1839466403 cites W1991221583 @default.
- W1839466403 cites W1991847459 @default.
- W1839466403 cites W2005072748 @default.
- W1839466403 cites W2007607723 @default.
- W1839466403 cites W2014455525 @default.
- W1839466403 cites W2016892218 @default.
- W1839466403 cites W2021506809 @default.
- W1839466403 cites W2021550972 @default.
- W1839466403 cites W2023296392 @default.
- W1839466403 cites W2024681370 @default.
- W1839466403 cites W2027885271 @default.
- W1839466403 cites W2028253963 @default.
- W1839466403 cites W2033272479 @default.
- W1839466403 cites W2033474014 @default.
- W1839466403 cites W2034184190 @default.
- W1839466403 cites W2037810763 @default.
- W1839466403 cites W2038800516 @default.
- W1839466403 cites W2042992678 @default.
- W1839466403 cites W2044391764 @default.
- W1839466403 cites W2044567633 @default.
- W1839466403 cites W2052098523 @default.
- W1839466403 cites W2052318709 @default.
- W1839466403 cites W2053293963 @default.
- W1839466403 cites W2059157898 @default.
- W1839466403 cites W2067897176 @default.
- W1839466403 cites W2070172803 @default.
- W1839466403 cites W2070815453 @default.
- W1839466403 cites W2072287141 @default.
- W1839466403 cites W2083652435 @default.
- W1839466403 cites W2102674766 @default.
- W1839466403 cites W2106607929 @default.
- W1839466403 cites W2106616036 @default.
- W1839466403 cites W2109351872 @default.
- W1839466403 cites W2109736922 @default.
- W1839466403 cites W2117035634 @default.
- W1839466403 cites W2137413193 @default.
- W1839466403 cites W2140784698 @default.
- W1839466403 cites W2149922101 @default.
- W1839466403 cites W2150373282 @default.
- W1839466403 cites W2154604772 @default.
- W1839466403 cites W2155339615 @default.
- W1839466403 cites W2163352549 @default.
- W1839466403 cites W2163567213 @default.
- W1839466403 cites W2167192225 @default.
- W1839466403 cites W2169119281 @default.
- W1839466403 doi "https://doi.org/10.1002/term.1843" @default.
- W1839466403 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4076346" @default.
- W1839466403 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24170301" @default.
- W1839466403 hasPublicationYear "2013" @default.
- W1839466403 type Work @default.
- W1839466403 sameAs 1839466403 @default.
- W1839466403 citedByCount "13" @default.
- W1839466403 countsByYear W18394664032015 @default.
- W1839466403 countsByYear W18394664032016 @default.
- W1839466403 countsByYear W18394664032017 @default.
- W1839466403 countsByYear W18394664032018 @default.
- W1839466403 countsByYear W18394664032019 @default.
- W1839466403 countsByYear W18394664032021 @default.
- W1839466403 countsByYear W18394664032022 @default.
- W1839466403 countsByYear W18394664032023 @default.
- W1839466403 crossrefType "journal-article" @default.
- W1839466403 hasAuthorship W1839466403A5033056518 @default.
- W1839466403 hasAuthorship W1839466403A5054766668 @default.
- W1839466403 hasAuthorship W1839466403A5058060254 @default.
- W1839466403 hasAuthorship W1839466403A5072121602 @default.
- W1839466403 hasAuthorship W1839466403A5086619153 @default.