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- W1839949658 abstract "A pressurized capillary electrochromatography (pCEC) method combined with an online concentration was developed for the separation and determination of five β2 -blockers (terbutaline, salbutamol, formoterol, procaterol and salmeterol) in human urine. A stearyl methacrylate-based monolithic column was prepared as the separation column. The various separation parameters including acetontrile concentration, applied voltage, pH and concentration of the running buffer were investigated. On-line concentration methods in combination of the chromatographic zone-sharpening effect and field-enhanced sample-stacking effect were utilized to improve detection sensitivity. The on-line concentration parameters including injection voltage, injection time, as well as sample matrix were systematically studied. Compared with the conventional sample injection, the on-line concentration technique appeared to improve the corresponding sensitivities 20∽50-fold. Furthermore, good precision was obtained with relative standard deviations (RSDs) for retention times within 1.09% and for peak areas less than 3.55% (n = 5). The established method was successfully applied to the determination of above-mentioned β2 -agonists in urine samples. The recoveries of spiked urine samples were between 85.0 and 113.3% with RSDs less than 5.39%." @default.
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- W1839949658 date "2015-09-07" @default.
- W1839949658 modified "2023-09-27" @default.
- W1839949658 title "On-line concentration and pressurized capillary electrochromatography analysis of five β-agonists in human urine using a methacrylate monolithic column" @default.
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- W1839949658 doi "https://doi.org/10.1002/elps.201500229" @default.
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