FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1840292617> ?p ?o ?g. }

• W1840292617 endingPage "901" @default.
• W1840292617 startingPage "891" @default.
• W1840292617 abstract "In this study we evaluate, for the first time, the molecular mechanism that underlies the extinction of a tissue-specific transcription factor, Oct-3/4, in somatic cell hybrids and compared it with its down-regulation in retinoic acid (RA)-treated embryonal carcinoma (EC) cells. The Oct-3/4 gene, which belongs to the POU family of transcription factors and is abundantly expressed in EC (OTF9-63) cells, provides an excellent model system with which to study the extinction phenomenon. Unlike other genes whose expression has been repressed in hybrid cells but not during in vivo differentiation, Oct-3/4 expression is dramatically repressed in OTF9-63 x fibroblast hybrids and also during embryogenesis. The ectopic expression of Oct-3/4 in hybrid cells under a constitutive promoter is sufficient for transcriptional activation of an octamer-dependent promoter. These results argue against the possibility that fibroblasts contain a direct repressor which binds directly to the octamer sequence and prevents Oct-3/4 protein from binding. The extinction of Oct-3/4 binding activity in the hybrid cells occurs at the level of mRNA transcription, similarly to the repression of Oct-3/4 transcription during in vivo differentiation. This shutdown of Oct-3/4 transcription in hybrid cells and in RA-treated EC cells is accompanied by de novo methylation of its 1.3-kb upstream region. In contrast to EC cells, in which this region is sensitive to MspI digestion, in hybrid cells and in RA-treated EC cells, the Oct-3/4 upstream region is resistant to MspI digestion, which suggests a change in its chromatin structure. Furthermore, extinction is not restricted to the endogenous Oct-3/4 gene but is also exerted upon a transiently transfected reporter gene driven by the Oct-3/4 upstream region. Thus, changes in the cellular activity of trans-acting factors acting on the upstream region also contribute to the inability of the hybrid and RA-treated EC cells to generate Oct-3/4 transcripts. In conclusion, this study draws a connection between the shutdown of Oct-3/4 expression in RA-differentiated EC cells and its extinction in hybrid cells. In both systems, repression of Oct-3/4 expression is achieved through changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream regulatory region." @default.
• W1840292617 created "2016-06-24" @default.
• W1840292617 creator A5007125099 @default.
• W1840292617 creator A5030366259 @default.
• W1840292617 creator A5048803548 @default.
• W1840292617 creator A5053388072 @default.
• W1840292617 date "1993-02-01" @default.
• W1840292617 modified "2023-10-15" @default.
• W1840292617 title "Extinction of Oct-3/4 gene expression in embryonal carcinoma x fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region." @default.
• W1840292617 cites W1444863543 @default.
• W1840292617 cites W1457782476 @default.
• W1840292617 cites W1514958964 @default.
• W1840292617 cites W1545735125 @default.
• W1840292617 cites W1571451935 @default.
• W1840292617 cites W167532633 @default.
• W1840292617 cites W1868383702 @default.
• W1840292617 cites W1954580706 @default.
• W1840292617 cites W1965267927 @default.
• W1840292617 cites W1971180826 @default.
• W1840292617 cites W1971983518 @default.
• W1840292617 cites W1975791071 @default.
• W1840292617 cites W1979981202 @default.
• W1840292617 cites W1981286265 @default.
• W1840292617 cites W1982884505 @default.
• W1840292617 cites W1985270394 @default.
• W1840292617 cites W1991198967 @default.
• W1840292617 cites W1993243592 @default.
• W1840292617 cites W1997317042 @default.
• W1840292617 cites W2003809571 @default.
• W1840292617 cites W2004144862 @default.
• W1840292617 cites W2004211510 @default.
• W1840292617 cites W2004563262 @default.
• W1840292617 cites W2004855923 @default.
• W1840292617 cites W2010356985 @default.
• W1840292617 cites W2023116564 @default.
• W1840292617 cites W2024449277 @default.
• W1840292617 cites W2027703149 @default.
• W1840292617 cites W2030083232 @default.
• W1840292617 cites W2032648102 @default.
• W1840292617 cites W2033162367 @default.
• W1840292617 cites W2033302775 @default.
• W1840292617 cites W2039826008 @default.
• W1840292617 cites W2040808575 @default.
• W1840292617 cites W2050951221 @default.
• W1840292617 cites W2056771313 @default.
• W1840292617 cites W2062362728 @default.
• W1840292617 cites W2063248559 @default.
• W1840292617 cites W2065071837 @default.
• W1840292617 cites W2081267992 @default.
• W1840292617 cites W2084099638 @default.
• W1840292617 cites W2084678798 @default.
• W1840292617 cites W2089475356 @default.
• W1840292617 cites W2096367521 @default.
• W1840292617 cites W2110050753 @default.
• W1840292617 cites W2114090617 @default.
• W1840292617 cites W2119658359 @default.
• W1840292617 cites W2128277573 @default.
• W1840292617 cites W2128635872 @default.
• W1840292617 cites W2140590723 @default.
• W1840292617 cites W2144206181 @default.
• W1840292617 cites W2144634347 @default.
• W1840292617 cites W2153782423 @default.
• W1840292617 cites W2156882409 @default.
• W1840292617 cites W2165135585 @default.
• W1840292617 cites W233481338 @default.
• W1840292617 cites W405995054 @default.
• W1840292617 cites W572814906 @default.
• W1840292617 cites W74964901 @default.
• W1840292617 doi "https://doi.org/10.1128/mcb.13.2.891" @default.
• W1840292617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358972" @default.
• W1840292617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7678695" @default.
• W1840292617 hasPublicationYear "1993" @default.
• W1840292617 type Work @default.
• W1840292617 sameAs 1840292617 @default.
• W1840292617 citedByCount "70" @default.
• W1840292617 countsByYear W18402926172012 @default.
• W1840292617 countsByYear W18402926172013 @default.
• W1840292617 countsByYear W18402926172014 @default.
• W1840292617 countsByYear W18402926172016 @default.
• W1840292617 countsByYear W18402926172019 @default.
• W1840292617 countsByYear W18402926172021 @default.
• W1840292617 countsByYear W18402926172022 @default.
• W1840292617 crossrefType "journal-article" @default.
• W1840292617 hasAuthorship W1840292617A5007125099 @default.
• W1840292617 hasAuthorship W1840292617A5030366259 @default.
• W1840292617 hasAuthorship W1840292617A5048803548 @default.
• W1840292617 hasAuthorship W1840292617A5053388072 @default.
• W1840292617 hasBestOaLocation W18402926171 @default.
• W1840292617 hasConcept C101762097 @default.
• W1840292617 hasConcept C104317684 @default.
• W1840292617 hasConcept C121587040 @default.
• W1840292617 hasConcept C134305767 @default.
• W1840292617 hasConcept C138885662 @default.
• W1840292617 hasConcept C150194340 @default.
• W1840292617 hasConcept C153911025 @default.
• W1840292617 hasConcept C158448853 @default.
• W1840292617 hasConcept C165864922 @default.
• W1840292617 hasConcept C179926584 @default.

• next