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- W184088107 abstract "This chapter discusses an experiment to study the expression of influenza virus subgenomic virion RNAs in infected cells. For the isolation of temperature-sensitive (ts) mutants that induce the synthesis of novel polypeptides in the experiment, six isolates of a ts mutant defective in RNA segment 1 were obtained by recombination between a double mutant of FPV Rostock, ts C47d, and the wild type virus. Each isolate was the product of a separate recombination experiment. RNA was extracted from CEF cells infected with tsC and translated in the wheat germ cell free translation system. For the dentification of tsC-specific subgenomic RNAs in the experiment, mRNAs from infected cells were identified by oligo-dT primed reverse transcription of RNA in the presence of an (α32P)-labeled deoxynucleoside triphosphate, followed by the selection of cDNAs by hybridization to plasmid DNA containing FPV Rostock-specific cDNA inserts." @default.
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- W184088107 date "1984-01-01" @default.
- W184088107 modified "2023-10-05" @default.
- W184088107 title "EXPRESSION OF INFLUENZA VIRUS SUBGENOMIC VIRION RNAs IN INFECTED CELLS" @default.
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- W184088107 doi "https://doi.org/10.1016/b978-0-12-183501-9.50029-7" @default.
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