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• W1840893585 abstract "Abstract : The formation of large, complex structures from small building blocks through self-assembly is widely seen in proteins and provides a tool for the creation of functional nanoscale devices. However, the factors controlling protein self-assembly are complex and often poorly understood. Peroxiredoxins are a large family of proteins, many of which are able to form a variety of large structures from a small, basic unit. This assembly has been shown to be strongly influenced by the redox state of the enzyme, which functions as a switch, controlling self-assembly. This thesis uses a protein from this family, human peroxiredoxin 3 (hPrx3) as a model system to investigate whether the self-assembly properties of hPrx3 can be influenced by rational protein engineering. Three forms of hPrx3 were purified and examined. These were the wild type and two variants: a mutant (S78A) and a His-tagged form. Size exclusion chromatography showed that each form showed a different ratio of dimers and larger species. Both variants showed preference for larger species, especially in the His-tagged form. This was shown to be partially dependent on metal binding in the His-tagged form. Larger species formed from multiple rings were also identified. SAXS measurement indicated that in the wild type enzyme, higher order species were dodecameric rings. For the His-tagged variant, SAXS measurement showed that the species observed had a different structure than that of the wild type. Electron microscopy showed that higher order structures seen in both wild type hPrx3 and His-tagged hPrx3 were ring shaped, with dimensions consistent with dodecamers. A competitive assay showed that the wild type, with kcat/km values near 2 107, consistent with published results. Both variant forms showed evidence of slightly higher activity than the wild type, indicating a link between activity and assembly." @default.
• W1840893585 created "2016-06-24" @default.
• W1840893585 creator A5073742113 @default.
• W1840893585 date "2014-08-24" @default.
• W1840893585 modified "2023-09-27" @default.
• W1840893585 title "Peroxiredoxins : a model for a self-assembling nanoscale system." @default.
• W1840893585 cites W111361069 @default.
• W1840893585 cites W1520425329 @default.
• W1840893585 cites W1563231805 @default.
• W1840893585 cites W1589043152 @default.
• W1840893585 cites W1634384702 @default.
• W1840893585 cites W1668019941 @default.
• W1840893585 cites W170763897 @default.
• W1840893585 cites W1775749144 @default.
• W1840893585 cites W1810600521 @default.
• W1840893585 cites W1925799544 @default.
• W1840893585 cites W1972875046 @default.
• W1840893585 cites W1974011753 @default.
• W1840893585 cites W1974658879 @default.
• W1840893585 cites W1974700900 @default.
• W1840893585 cites W1976006396 @default.
• W1840893585 cites W1977793339 @default.
• W1840893585 cites W1978874279 @default.
• W1840893585 cites W1979309046 @default.
• W1840893585 cites W1979701801 @default.
• W1840893585 cites W1981112109 @default.
• W1840893585 cites W1981473145 @default.
• W1840893585 cites W1981627979 @default.
• W1840893585 cites W1981968407 @default.
• W1840893585 cites W1983316037 @default.
• W1840893585 cites W1984060522 @default.
• W1840893585 cites W1986508375 @default.
• W1840893585 cites W1989400319 @default.
• W1840893585 cites W1991952977 @default.
• W1840893585 cites W1997566863 @default.
• W1840893585 cites W1999377438 @default.
• W1840893585 cites W1999382188 @default.
• W1840893585 cites W2000388754 @default.
• W1840893585 cites W2003589493 @default.
• W1840893585 cites W2004128120 @default.
• W1840893585 cites W2004617250 @default.
• W1840893585 cites W2005579255 @default.
• W1840893585 cites W2006225923 @default.
• W1840893585 cites W2006723796 @default.
• W1840893585 cites W2007684558 @default.
• W1840893585 cites W2009284411 @default.
• W1840893585 cites W2010218425 @default.
• W1840893585 cites W2012763468 @default.
• W1840893585 cites W2015970511 @default.
• W1840893585 cites W2016894211 @default.
• W1840893585 cites W2017642315 @default.
• W1840893585 cites W2019560775 @default.
• W1840893585 cites W2020400942 @default.
• W1840893585 cites W2022504555 @default.
• W1840893585 cites W2022561948 @default.
• W1840893585 cites W2023231364 @default.
• W1840893585 cites W2026225426 @default.
• W1840893585 cites W2033534904 @default.
• W1840893585 cites W2033800625 @default.
• W1840893585 cites W2033832729 @default.
• W1840893585 cites W2036603507 @default.
• W1840893585 cites W2037083980 @default.
• W1840893585 cites W2049713754 @default.
• W1840893585 cites W2051687003 @default.
• W1840893585 cites W2051710717 @default.
• W1840893585 cites W2053219282 @default.
• W1840893585 cites W2054620060 @default.
• W1840893585 cites W205714514 @default.
• W1840893585 cites W2057857670 @default.
• W1840893585 cites W2062901635 @default.
• W1840893585 cites W2064680209 @default.
• W1840893585 cites W2065427954 @default.
• W1840893585 cites W2066088601 @default.
• W1840893585 cites W2066327917 @default.
• W1840893585 cites W2066639890 @default.
• W1840893585 cites W2067748586 @default.
• W1840893585 cites W2069507241 @default.
• W1840893585 cites W2069520354 @default.
• W1840893585 cites W2070783701 @default.
• W1840893585 cites W2072132202 @default.
• W1840893585 cites W2072232369 @default.
• W1840893585 cites W2073937912 @default.
• W1840893585 cites W2075584603 @default.
• W1840893585 cites W2077463476 @default.
• W1840893585 cites W2078672958 @default.
• W1840893585 cites W2082318697 @default.
• W1840893585 cites W2084065253 @default.
• W1840893585 cites W2084949662 @default.
• W1840893585 cites W2087098118 @default.
• W1840893585 cites W2087369660 @default.
• W1840893585 cites W2088411222 @default.
• W1840893585 cites W2088564722 @default.
• W1840893585 cites W2092463196 @default.
• W1840893585 cites W2098048169 @default.
• W1840893585 cites W2100600786 @default.
• W1840893585 cites W2101926688 @default.
• W1840893585 cites W2106320506 @default.
• W1840893585 cites W2110286022 @default.
• W1840893585 cites W2113373875 @default.
• W1840893585 cites W2117418022 @default.

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