FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1840965475> ?p ?o ?g. }

• W1840965475 endingPage "626" @default.
• W1840965475 startingPage "618" @default.
• W1840965475 abstract "Hydrophobic interaction chromatography (HIC) is an important tool in the industrial purification of proteins from various sources. The HIC separation behavior of individual (or model) proteins has been widely researched by others. On the contrary, this study focused on the fractionation ability of HIC when it is challenged with whole proteomes. The impact of the nature of three different proteomes, that is, yeast, soybean, and Chinese hamster ovary cells, on HIC separation was investigated. In doing so, chromatography fractions obtained under standardized conditions were evaluated in terms of their overall hydrophobicity—as measured by fluorescence dye binding. This technique allowed for the calculation of an average protein surface hydrophobicity (S 0 ) for each fraction; a unique correlation between S 0 and the observed chromatographic behavior was established in each case. Following a similar strategy, the effect of three different ligands (polypropylene glycol, phenyl, and butyl) and two adsorbent particle sizes (65 and 100 µm) on the chromatographic behavior of the yeast proteome was evaluated. As expected, the superficial hydrophobicity of the proteins eluted is correlated with the salt concentration of its corresponding elution step. The findings reveled how—and in which extent—the type of ligand and the size of the beads actually influenced the fractionation of the complex biological mixture. Summarizing, the approach presented here can be instrumental to the study of the performance of chromatography adsorbents under conditions close to industrial practice and to the development of downstream processing strategies. Copyright © 2013 John Wiley & Sons, Ltd." @default.
• W1840965475 created "2016-06-24" @default.
• W1840965475 creator A5008533838 @default.
• W1840965475 creator A5009807307 @default.
• W1840965475 creator A5075128199 @default.
• W1840965475 creator A5089373546 @default.
• W1840965475 date "2013-11-11" @default.
• W1840965475 modified "2023-10-10" @default.
• W1840965475 title "Proteome wide evaluation of the separation ability of hydrophobic interaction chromatography by fluorescent dye binding analysis" @default.
• W1840965475 cites W138424610 @default.
• W1840965475 cites W1964206438 @default.
• W1840965475 cites W1964347315 @default.
• W1840965475 cites W1967681366 @default.
• W1840965475 cites W1968469489 @default.
• W1840965475 cites W1968518086 @default.
• W1840965475 cites W1968913285 @default.
• W1840965475 cites W1973087371 @default.
• W1840965475 cites W1974098644 @default.
• W1840965475 cites W1976697284 @default.
• W1840965475 cites W1977013674 @default.
• W1840965475 cites W1994125987 @default.
• W1840965475 cites W1994812138 @default.
• W1840965475 cites W1994992178 @default.
• W1840965475 cites W1996721996 @default.
• W1840965475 cites W2007209382 @default.
• W1840965475 cites W2011067214 @default.
• W1840965475 cites W2025402276 @default.
• W1840965475 cites W2032812903 @default.
• W1840965475 cites W2044246569 @default.
• W1840965475 cites W2047841570 @default.
• W1840965475 cites W2053352111 @default.
• W1840965475 cites W2069199036 @default.
• W1840965475 cites W2071875593 @default.
• W1840965475 cites W2074182486 @default.
• W1840965475 cites W2082026326 @default.
• W1840965475 cites W2082467946 @default.
• W1840965475 cites W2093172125 @default.
• W1840965475 cites W2149501357 @default.
• W1840965475 cites W2333998345 @default.
• W1840965475 cites W4242647508 @default.
• W1840965475 cites W4293247451 @default.
• W1840965475 cites W82018583 @default.
• W1840965475 doi "https://doi.org/10.1002/jmr.2302" @default.
• W1840965475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24277606" @default.
• W1840965475 hasPublicationYear "2013" @default.
• W1840965475 type Work @default.
• W1840965475 sameAs 1840965475 @default.
• W1840965475 citedByCount "2" @default.
• W1840965475 countsByYear W18409654752016 @default.
• W1840965475 countsByYear W18409654752021 @default.
• W1840965475 crossrefType "journal-article" @default.
• W1840965475 hasAuthorship W1840965475A5008533838 @default.
• W1840965475 hasAuthorship W1840965475A5009807307 @default.
• W1840965475 hasAuthorship W1840965475A5075128199 @default.
• W1840965475 hasAuthorship W1840965475A5089373546 @default.
• W1840965475 hasConcept C104397665 @default.
• W1840965475 hasConcept C116817701 @default.
• W1840965475 hasConcept C121332964 @default.
• W1840965475 hasConcept C150394285 @default.
• W1840965475 hasConcept C178790620 @default.
• W1840965475 hasConcept C179998833 @default.
• W1840965475 hasConcept C185592680 @default.
• W1840965475 hasConcept C2779222958 @default.
• W1840965475 hasConcept C43617362 @default.
• W1840965475 hasConcept C55493867 @default.
• W1840965475 hasConcept C62520636 @default.
• W1840965475 hasConcept C73826308 @default.
• W1840965475 hasConcept C90260826 @default.
• W1840965475 hasConcept C91881484 @default.
• W1840965475 hasConcept C97428945 @default.
• W1840965475 hasConceptScore W1840965475C104397665 @default.
• W1840965475 hasConceptScore W1840965475C116817701 @default.
• W1840965475 hasConceptScore W1840965475C121332964 @default.
• W1840965475 hasConceptScore W1840965475C150394285 @default.
• W1840965475 hasConceptScore W1840965475C178790620 @default.
• W1840965475 hasConceptScore W1840965475C179998833 @default.
• W1840965475 hasConceptScore W1840965475C185592680 @default.
• W1840965475 hasConceptScore W1840965475C2779222958 @default.
• W1840965475 hasConceptScore W1840965475C43617362 @default.
• W1840965475 hasConceptScore W1840965475C55493867 @default.
• W1840965475 hasConceptScore W1840965475C62520636 @default.
• W1840965475 hasConceptScore W1840965475C73826308 @default.
• W1840965475 hasConceptScore W1840965475C90260826 @default.
• W1840965475 hasConceptScore W1840965475C91881484 @default.
• W1840965475 hasConceptScore W1840965475C97428945 @default.
• W1840965475 hasIssue "12" @default.
• W1840965475 hasLocation W18409654751 @default.
• W1840965475 hasLocation W18409654752 @default.
• W1840965475 hasOpenAccess W1840965475 @default.
• W1840965475 hasPrimaryLocation W18409654751 @default.
• W1840965475 hasRelatedWork W1994125987 @default.
• W1840965475 hasRelatedWork W2036706461 @default.
• W1840965475 hasRelatedWork W2050056464 @default.
• W1840965475 hasRelatedWork W2059148516 @default.
• W1840965475 hasRelatedWork W2550622545 @default.
• W1840965475 hasRelatedWork W2739960335 @default.
• W1840965475 hasRelatedWork W2977145366 @default.
• W1840965475 hasRelatedWork W4205173269 @default.

• next