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- W1841192023 abstract "Stalheim , O. H. V. (University of Wisconsin, Madison), and J. B. Wilson . Cultivation of leptospirae. II. Growth and lysis in synthetic medium. J. Bacteriol. 88: 55–59. 1964.—Differences were found in the ability of leptospirae to grow in a synthetic medium; 43 strains, consisting of 16 serotypes, were tested and designated as either type I or type II. Type I leptospirae did not grow; type II grew and could be subcultured. The lytic effect of several lipids was measured with Leptospira pomona and L. canicola as representatives of type I and II leptospirae, respectively. L. pomona organisms were rapidly lysed by the monoolein of the synthetic medium and by other lipids as well; L. canicola cells were consistently more resistant. Although both organisms incorporated similar amounts of label when incubated in the presence of oleic- 1-C 14 acid, only L. canicola grew in a modified, nonlytic synthetic medium. No differences were found in susceptibility to lysis between virulent and avirulent L. canicola organisms. Mutant type I leptospirae grown in synthetic medium had increased resistance to lysis by surface-active agents; they were poorly agglutinated by antiserum. The role of protein in the growth and antigenicity of type I leptospirae is discussed." @default.
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- W1841192023 date "1964-07-01" @default.
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- W1841192023 title "CULTIVATION OF LEPTOSPIRAE II" @default.
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- W1841192023 doi "https://doi.org/10.1128/jb.88.1.55-59.1964" @default.
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