Matches in SemOpenAlex for { <https://semopenalex.org/work/W1842542023> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1842542023 endingPage "7443" @default.
- W1842542023 startingPage "7438" @default.
- W1842542023 abstract "Three cDNA clones comprising the VP8 subunit of the VP4 of human rotavirus strain KU (VP7 serotype G1; VP4 serotype P1A) G1 were constructed. The corresponding encoded peptides were designated according to their locations in the VP8 subunit as A (amino acids 1 to 102), B (amino acids 84 to 180), and C (amino acids 150 to 246 plus amino acids 247 to 251 from VP5). In addition, cDNA clones encoding peptide B of the VP8 subunit of the VP4 gene from human rotavirus strains DS-1 (G2; P1B) and 1076 (G2; P2) were also constructed. These DNA fragments were inserted into plasmid pGEMEX-1 and expressed in Escherichia coli. Western immunoblot analysis using antisera to rotavirus strains KU (P1A), Wa (P1A), DS-1 (P1B), 1076 (P2), and M37 (P2) demonstrated that peptides A and C cross-reacted with heterotypic human rotavirus VP4 antisera, suggesting that these two peptides represent conserved epitopes in the VP8 subunit. In contrast, peptide B appears to be involved in the VP4 serotype and subtype specificities, because it reacted only with the corresponding serotype- and subtype-specific antiserum. Antiserum raised against peptide A, B, or C of strain KU contained a lower level of neutralizing activity than did that induced by the entire VP8 subunit. In addition, the serotype-specific neutralizing activity of anti-KU VP8 serum was ablated after adsorption with the KU VP8 protein but not with a mixture of peptides A, B, and C of strain KU, suggesting that most of the serotype-specific epitopes in the VP8 subunit are conformational and are dependent on the entire amino acid sequence of VP8." @default.
- W1842542023 created "2016-06-24" @default.
- W1842542023 creator A5006945218 @default.
- W1842542023 creator A5044934436 @default.
- W1842542023 date "1992-12-01" @default.
- W1842542023 modified "2023-09-27" @default.
- W1842542023 title "Distribution of conserved and specific epitopes on the VP8 subunit of rotavirus VP4" @default.
- W1842542023 cites W1516325674 @default.
- W1842542023 cites W1575279099 @default.
- W1842542023 cites W1598891130 @default.
- W1842542023 cites W1960193626 @default.
- W1842542023 cites W1975993749 @default.
- W1842542023 cites W1988463994 @default.
- W1842542023 cites W2003966164 @default.
- W1842542023 cites W2015056320 @default.
- W1842542023 cites W2018927042 @default.
- W1842542023 cites W2027433479 @default.
- W1842542023 cites W2041301110 @default.
- W1842542023 cites W2050717506 @default.
- W1842542023 cites W2051223657 @default.
- W1842542023 cites W2058621010 @default.
- W1842542023 cites W2093835870 @default.
- W1842542023 cites W2145342678 @default.
- W1842542023 doi "https://doi.org/10.1128/jvi.66.12.7438-7443.1992" @default.
- W1842542023 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/240451" @default.
- W1842542023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1279204" @default.
- W1842542023 hasPublicationYear "1992" @default.
- W1842542023 type Work @default.
- W1842542023 sameAs 1842542023 @default.
- W1842542023 citedByCount "33" @default.
- W1842542023 countsByYear W18425420232012 @default.
- W1842542023 countsByYear W18425420232015 @default.
- W1842542023 countsByYear W18425420232016 @default.
- W1842542023 countsByYear W18425420232018 @default.
- W1842542023 crossrefType "journal-article" @default.
- W1842542023 hasAuthorship W1842542023A5006945218 @default.
- W1842542023 hasAuthorship W1842542023A5044934436 @default.
- W1842542023 hasBestOaLocation W18425420231 @default.
- W1842542023 hasConcept C10389963 @default.
- W1842542023 hasConcept C104292427 @default.
- W1842542023 hasConcept C104317684 @default.
- W1842542023 hasConcept C153911025 @default.
- W1842542023 hasConcept C159047783 @default.
- W1842542023 hasConcept C159654299 @default.
- W1842542023 hasConcept C167625842 @default.
- W1842542023 hasConcept C187882448 @default.
- W1842542023 hasConcept C195616568 @default.
- W1842542023 hasConcept C2522874641 @default.
- W1842542023 hasConcept C2779281246 @default.
- W1842542023 hasConcept C2779416561 @default.
- W1842542023 hasConcept C2781367151 @default.
- W1842542023 hasConcept C515207424 @default.
- W1842542023 hasConcept C54355233 @default.
- W1842542023 hasConcept C55493867 @default.
- W1842542023 hasConcept C86803240 @default.
- W1842542023 hasConcept C91523082 @default.
- W1842542023 hasConceptScore W1842542023C10389963 @default.
- W1842542023 hasConceptScore W1842542023C104292427 @default.
- W1842542023 hasConceptScore W1842542023C104317684 @default.
- W1842542023 hasConceptScore W1842542023C153911025 @default.
- W1842542023 hasConceptScore W1842542023C159047783 @default.
- W1842542023 hasConceptScore W1842542023C159654299 @default.
- W1842542023 hasConceptScore W1842542023C167625842 @default.
- W1842542023 hasConceptScore W1842542023C187882448 @default.
- W1842542023 hasConceptScore W1842542023C195616568 @default.
- W1842542023 hasConceptScore W1842542023C2522874641 @default.
- W1842542023 hasConceptScore W1842542023C2779281246 @default.
- W1842542023 hasConceptScore W1842542023C2779416561 @default.
- W1842542023 hasConceptScore W1842542023C2781367151 @default.
- W1842542023 hasConceptScore W1842542023C515207424 @default.
- W1842542023 hasConceptScore W1842542023C54355233 @default.
- W1842542023 hasConceptScore W1842542023C55493867 @default.
- W1842542023 hasConceptScore W1842542023C86803240 @default.
- W1842542023 hasConceptScore W1842542023C91523082 @default.
- W1842542023 hasIssue "12" @default.
- W1842542023 hasLocation W18425420231 @default.
- W1842542023 hasLocation W18425420232 @default.
- W1842542023 hasLocation W18425420233 @default.
- W1842542023 hasLocation W18425420234 @default.
- W1842542023 hasOpenAccess W1842542023 @default.
- W1842542023 hasPrimaryLocation W18425420231 @default.
- W1842542023 hasRelatedWork W1522005625 @default.
- W1842542023 hasRelatedWork W1927161870 @default.
- W1842542023 hasRelatedWork W1927508411 @default.
- W1842542023 hasRelatedWork W1961489291 @default.
- W1842542023 hasRelatedWork W1965095067 @default.
- W1842542023 hasRelatedWork W1977346347 @default.
- W1842542023 hasRelatedWork W1984953318 @default.
- W1842542023 hasRelatedWork W1987645034 @default.
- W1842542023 hasRelatedWork W2008805361 @default.
- W1842542023 hasRelatedWork W2026673299 @default.
- W1842542023 hasVolume "66" @default.
- W1842542023 isParatext "false" @default.
- W1842542023 isRetracted "false" @default.
- W1842542023 magId "1842542023" @default.
- W1842542023 workType "article" @default.