Matches in SemOpenAlex for { <https://semopenalex.org/work/W1843332974> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1843332974 endingPage "2769" @default.
- W1843332974 startingPage "2765" @default.
- W1843332974 abstract "In the present investigation, explants from Kalanchoë blossfeldiana were used for gene transformation. The young leaves were inoculated with Agrobacterium tumefaciens LBA4404 strain with a binary vector plasmid pArtblue containing F3'5'H gene under control of CaMV35S promoter and nptII selectable marker gene. After inoculation, the explants were transferred to the co-cultivation medium. They were then transferred to the selection medium containing kanamycin and were sub-cultured every two weeks. Leaves of the putative transgenic shoots that survived in the selection medium were used in reverse transcription polymerase chain reaction (RT-PCR) analysis to detect gene expression. The RT-PCR analysis showed the presence of 550 bp F3'5'H amplification products and had an expression of F3'5'H gene. Plants with the introduced F3'5'H gene produced totally pale red flowers. Key words: Kalanchoë blossfeldiana, Agrobacterium-mediated transformation, young leaf, F3'5'H gene, reverse transcription-polymerase chain reaction (RT-PCR)." @default.
- W1843332974 created "2016-06-24" @default.
- W1843332974 creator A5047877161 @default.
- W1843332974 creator A5051503821 @default.
- W1843332974 creator A5079648771 @default.
- W1843332974 date "2015-09-30" @default.
- W1843332974 modified "2023-10-03" @default.
- W1843332974 title "A protocol for Agrobacterium-mediated transformation of Kalancho blossfeldiana with a flavonoid 3,5 hydroxylase (F35H) gene" @default.
- W1843332974 cites W1575500725 @default.
- W1843332974 cites W1661448904 @default.
- W1843332974 cites W184652191 @default.
- W1843332974 cites W1966038316 @default.
- W1843332974 cites W1976061210 @default.
- W1843332974 cites W1993947614 @default.
- W1843332974 cites W1994170944 @default.
- W1843332974 cites W2004444907 @default.
- W1843332974 cites W2018429609 @default.
- W1843332974 cites W2024556021 @default.
- W1843332974 cites W2091070751 @default.
- W1843332974 cites W2099477241 @default.
- W1843332974 cites W2107330252 @default.
- W1843332974 cites W2116807706 @default.
- W1843332974 cites W2122967180 @default.
- W1843332974 cites W2127497651 @default.
- W1843332974 cites W2128912763 @default.
- W1843332974 cites W2129788711 @default.
- W1843332974 cites W2129051678 @default.
- W1843332974 doi "https://doi.org/10.5897/ajb2015.14854" @default.
- W1843332974 hasPublicationYear "2015" @default.
- W1843332974 type Work @default.
- W1843332974 sameAs 1843332974 @default.
- W1843332974 citedByCount "0" @default.
- W1843332974 crossrefType "journal-article" @default.
- W1843332974 hasAuthorship W1843332974A5047877161 @default.
- W1843332974 hasAuthorship W1843332974A5051503821 @default.
- W1843332974 hasAuthorship W1843332974A5079648771 @default.
- W1843332974 hasBestOaLocation W18433329741 @default.
- W1843332974 hasConcept C102230213 @default.
- W1843332974 hasConcept C104317684 @default.
- W1843332974 hasConcept C122172344 @default.
- W1843332974 hasConcept C150194340 @default.
- W1843332974 hasConcept C153911025 @default.
- W1843332974 hasConcept C157890192 @default.
- W1843332974 hasConcept C202751555 @default.
- W1843332974 hasConcept C204241405 @default.
- W1843332974 hasConcept C22744801 @default.
- W1843332974 hasConcept C2776162087 @default.
- W1843332974 hasConcept C2779656422 @default.
- W1843332974 hasConcept C2780920689 @default.
- W1843332974 hasConcept C33161422 @default.
- W1843332974 hasConcept C40767141 @default.
- W1843332974 hasConcept C42411736 @default.
- W1843332974 hasConcept C49105822 @default.
- W1843332974 hasConcept C54355233 @default.
- W1843332974 hasConcept C86803240 @default.
- W1843332974 hasConceptScore W1843332974C102230213 @default.
- W1843332974 hasConceptScore W1843332974C104317684 @default.
- W1843332974 hasConceptScore W1843332974C122172344 @default.
- W1843332974 hasConceptScore W1843332974C150194340 @default.
- W1843332974 hasConceptScore W1843332974C153911025 @default.
- W1843332974 hasConceptScore W1843332974C157890192 @default.
- W1843332974 hasConceptScore W1843332974C202751555 @default.
- W1843332974 hasConceptScore W1843332974C204241405 @default.
- W1843332974 hasConceptScore W1843332974C22744801 @default.
- W1843332974 hasConceptScore W1843332974C2776162087 @default.
- W1843332974 hasConceptScore W1843332974C2779656422 @default.
- W1843332974 hasConceptScore W1843332974C2780920689 @default.
- W1843332974 hasConceptScore W1843332974C33161422 @default.
- W1843332974 hasConceptScore W1843332974C40767141 @default.
- W1843332974 hasConceptScore W1843332974C42411736 @default.
- W1843332974 hasConceptScore W1843332974C49105822 @default.
- W1843332974 hasConceptScore W1843332974C54355233 @default.
- W1843332974 hasConceptScore W1843332974C86803240 @default.
- W1843332974 hasIssue "39" @default.
- W1843332974 hasLocation W18433329741 @default.
- W1843332974 hasOpenAccess W1843332974 @default.
- W1843332974 hasPrimaryLocation W18433329741 @default.
- W1843332974 hasRelatedWork W166275867 @default.
- W1843332974 hasRelatedWork W1986897237 @default.
- W1843332974 hasRelatedWork W2037198426 @default.
- W1843332974 hasRelatedWork W2148191914 @default.
- W1843332974 hasRelatedWork W2184352435 @default.
- W1843332974 hasRelatedWork W2394096477 @default.
- W1843332974 hasRelatedWork W2463309393 @default.
- W1843332974 hasRelatedWork W2555041069 @default.
- W1843332974 hasRelatedWork W4312871042 @default.
- W1843332974 hasRelatedWork W3033974903 @default.
- W1843332974 hasVolume "14" @default.
- W1843332974 isParatext "false" @default.
- W1843332974 isRetracted "false" @default.
- W1843332974 magId "1843332974" @default.
- W1843332974 workType "article" @default.