Matches in SemOpenAlex for { <https://semopenalex.org/work/W1843887216> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W1843887216 endingPage "141" @default.
- W1843887216 startingPage "141" @default.
- W1843887216 abstract "In both murine and porcine preimplantation stage clones, mosaicism in gene expression has been observed, indicating variation in transcription of some genes between cells of the individual clone (Boiani M et al., 2002 Genes Dev. 16, 1209–1219; Park KW et al., 2002 Biol. Reprod. 66, 1001–1005). This observation raises the question as to whether all blastomeres within one early-stage clone are equivalent, or whether there are differences in developmental potential. To address this, we aggregated preimplantation-stage clone embryos with fertilized embryos and assessed contribution of Oct4-GFP expressing cells of clone origin in blastocysts and in vitro outgrowths. In normal embryos, the Oct4-GFP transgene is expressed during preimplantation stages and reflects expression of Oct4 protein. Mouse cumulus cell clones were produced from cells transgenic for Oct4-GFP (Szabó PE et al., 2002 Mech. Dev. 115, 157–160) as described (Boiani M et al., 2002 Genes Dev. 16, 1209–1219). Four-cell-stage clones and synchronous fertilized non-transgenic embryos were aggregated in micro-wells after removal of the zona pellucida using acid Tyrode’s solution. Aggregates were cultured to the blastocyst stage in -MEM supplemented with bovine serum albumin (0.4% w/v). All control chimeras produced from four-cell-stage fertilized non-transgenic and Oct4-GFP transgenic embryos formed blastocysts, and 15 of 20 had GFP-expressing cells. The majority of clone-wild-type aggregates developed to the blastocyst stage (35/40); however, contribution of GFP-expressing cells was observed in fewer blastocysts compared to controls (12/35; P<0.05). Contribution of GFP expressing clone cells to the ICM varied between 30% and 100% of cells as determined by subjective evaluation of GFP fluorescence overlaying bright-field images. During in vitro outgrowth formation of synchronous aggregation chimeras of clone and wild-type embryos, maintenance of clone contribution to the ICM mound was observed, but at a lower frequency (12% v. 34% at the blastocyst stage). The results suggest that aggregation with fertilized cells does not provide benefit to clone blastomeres during preimplantation stages. Possibly, clone blastomeres may not be competitive with wild-type blastomeres, or are developmentally asynchronous, which will be tested using asynchronous chimeras." @default.
- W1843887216 created "2016-06-24" @default.
- W1843887216 creator A5012783591 @default.
- W1843887216 creator A5037447718 @default.
- W1843887216 creator A5045516752 @default.
- W1843887216 date "2004-01-01" @default.
- W1843887216 modified "2023-10-14" @default.
- W1843887216 title "37DEVELOPMENTAL POTENTIAL OF CLONE CELLS IN MURINE CLONE-FERTILIZED AGGREGATION CHIMERAS" @default.
- W1843887216 doi "https://doi.org/10.1071/rdv16n1ab37" @default.
- W1843887216 hasPublicationYear "2004" @default.
- W1843887216 type Work @default.
- W1843887216 sameAs 1843887216 @default.
- W1843887216 citedByCount "1" @default.
- W1843887216 countsByYear W18438872162022 @default.
- W1843887216 crossrefType "journal-article" @default.
- W1843887216 hasAuthorship W1843887216A5012783591 @default.
- W1843887216 hasAuthorship W1843887216A5037447718 @default.
- W1843887216 hasAuthorship W1843887216A5045516752 @default.
- W1843887216 hasBestOaLocation W18438872161 @default.
- W1843887216 hasConcept C102230213 @default.
- W1843887216 hasConcept C104317684 @default.
- W1843887216 hasConcept C142613039 @default.
- W1843887216 hasConcept C153911025 @default.
- W1843887216 hasConcept C16685009 @default.
- W1843887216 hasConcept C196843134 @default.
- W1843887216 hasConcept C2778177303 @default.
- W1843887216 hasConcept C30278631 @default.
- W1843887216 hasConcept C54355233 @default.
- W1843887216 hasConcept C68760520 @default.
- W1843887216 hasConcept C71924100 @default.
- W1843887216 hasConcept C81089528 @default.
- W1843887216 hasConcept C86803240 @default.
- W1843887216 hasConcept C87073359 @default.
- W1843887216 hasConceptScore W1843887216C102230213 @default.
- W1843887216 hasConceptScore W1843887216C104317684 @default.
- W1843887216 hasConceptScore W1843887216C142613039 @default.
- W1843887216 hasConceptScore W1843887216C153911025 @default.
- W1843887216 hasConceptScore W1843887216C16685009 @default.
- W1843887216 hasConceptScore W1843887216C196843134 @default.
- W1843887216 hasConceptScore W1843887216C2778177303 @default.
- W1843887216 hasConceptScore W1843887216C30278631 @default.
- W1843887216 hasConceptScore W1843887216C54355233 @default.
- W1843887216 hasConceptScore W1843887216C68760520 @default.
- W1843887216 hasConceptScore W1843887216C71924100 @default.
- W1843887216 hasConceptScore W1843887216C81089528 @default.
- W1843887216 hasConceptScore W1843887216C86803240 @default.
- W1843887216 hasConceptScore W1843887216C87073359 @default.
- W1843887216 hasIssue "2" @default.
- W1843887216 hasLocation W18438872161 @default.
- W1843887216 hasOpenAccess W1843887216 @default.
- W1843887216 hasPrimaryLocation W18438872161 @default.
- W1843887216 hasRelatedWork W1545105720 @default.
- W1843887216 hasRelatedWork W1963981764 @default.
- W1843887216 hasRelatedWork W1970916168 @default.
- W1843887216 hasRelatedWork W2040713823 @default.
- W1843887216 hasRelatedWork W2112979133 @default.
- W1843887216 hasRelatedWork W2119419499 @default.
- W1843887216 hasRelatedWork W2155524192 @default.
- W1843887216 hasRelatedWork W2375224314 @default.
- W1843887216 hasRelatedWork W2378797857 @default.
- W1843887216 hasRelatedWork W2990627236 @default.
- W1843887216 hasVolume "16" @default.
- W1843887216 isParatext "false" @default.
- W1843887216 isRetracted "false" @default.
- W1843887216 magId "1843887216" @default.
- W1843887216 workType "article" @default.