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- W184403785 abstract "Recently, it has been shown that cellular proto-oncogenes can be activated by single-base mutations. In addition, there is evidence to suggest that the nature of the activating mutation may be a consequence of specific chemical carcinogen-DNA interaction. Therefore, the authors sought to determine whether a relationship exists between PAH-DNA chemistry and chemically-induced genetic changes in end-stage liver tumors. Recently, they have demonstrated that a flounder c-Ki-{und ras} oncogene was present in NIH3T3 mouse fibroblasts transfected with DNA derived from a flounder liver tumor. Therefore, a more systematic study to detect oncogenes in affected flounder liver samples was undertaken. To characterize the oncogenes, DNA from the Nu-Nu tumors was isolated and analyzed by Southern blot hybridization of tumor DNA with a viral Ki-{und ras} DNA probe. This analysis revealed the presence of c-Ki-{und ras} oncogenes of fish origin in 6/7 (86%) of the Nu-Nu tumor DNAs analyzed. They hypothesize that common mechanisms of oncogene activation may be associated with tumor initiation. Using polymerase chain reaction (PCR) amplification and subsequent direct DNA sequence analysis of PCR-amplified DNA, they analyzed primary liver samples for the presence of DNA sequence changes in the first exon of the flounder c-Ki-{und ras} proto-oncogene. They have determinedmore » that a single G-C to T-A base transversion in the second position of the 12th codon (GLY to VAL) is present in a flounder sample which showed transforming activity in the Nu-Nu assay. This finding suggests that such a mutation may result in a transforming p21 protein in the flounder samples which were positive in the tumorgenicity assay.« less" @default.
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- W184403785 date "1988-09-01" @default.
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- W184403785 title "Characterization of C-Ki- und ras oncogenes in liver neoplasms of winter flounder from Boston Harbor" @default.
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